重组β-葡萄糖苷酶将人参皂苷Rf转化为人参皂苷Rh1

Ruan Chang-Chun, Zhang Hao, Zhang Lian-Xue, Liu Zhi, Sun Guang-Zhi, Lei Jun, Qin Yu-Xia, Zheng Yi-Nan, Li Xiang, Pan Hong-Yu

College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China.

Molecules. 2009 Jun 8;14(6):2043-8. doi: 10.3390/molecules14062043.

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (approximately 170 kD) showed the ability to transform ginsenoside Rf into Rh(1).

从人参果周围土壤中分离出一株黑曲霉。体外酶活性测定表明，该菌株具有将总人参皂苷（TGS）转化为几种新产物的能力。在进一步的生化研究中，从该菌株中分离出的β-葡萄糖苷酶基因bgl1在酿酒酵母中表达。带有组氨酸标签的BGL1蛋白（约170 kD）显示出将人参皂苷Rf转化为Rh(1)的能力。