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与Gen-Probe结核分枝杆菌扩增直接检测法和GenoType MTBDRplus相比,评估GenoType分枝杆菌直接检测法在临床样本中直接检测结核分枝杆菌复合群的效果。

Evaluation of GenoType mycobacteria direct assay in comparison with Gen-Probe Mycobacterium tuberculosis amplified direct test and GenoType MTBDRplus for direct detection of Mycobacterium tuberculosis complex in clinical samples.

作者信息

Neonakis Ioannis K, Gitti Zoe, Baritaki Stavroula, Petinaki Efi, Baritaki Maria, Spandidos Demetrios A

机构信息

Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Crete, Greece.

出版信息

J Clin Microbiol. 2009 Aug;47(8):2601-3. doi: 10.1128/JCM.02351-08. Epub 2009 Jun 24.

Abstract

Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplified Direct Test, 89.6%. The specificity of the assays used was 100%.

摘要

对三种分子检测方法进行了评估,以直接检测125份呼吸道样本和22份非呼吸道样本中的结核分枝杆菌复合群细菌。获得的总体灵敏度如下:GenoType MTBDRplus为97.9%;GenoType Mycobacteria Direct为93.7%;基因探针结核分枝杆菌扩增直接检测法为89.6%。所使用检测方法的特异性为100%。

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Molecular diagnostic tools in mycobacteriology.分枝杆菌学中的分子诊断工具。
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