State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Arch Oral Biol. 2009 Sep;54(9):817-22. doi: 10.1016/j.archoralbio.2009.06.007. Epub 2009 Jul 18.
This in vitro study was undertaken to assess the effects of Galla chinensis extract on inhibition of enamel caries-like demineralization and to elucidate the role of the organic matrix of enamel in this process.
Either regular or enamel disposed of its organic matrix both of bovine origin were exposed to a demineralizing solution for 3 days (pH 4.5). Specimes were additionally treated with either 4 g/L of G. chinensis extraction (GCE) or double distilled water (DDW) four times daily for 5 min each time. Regular enamel exposed accordingly to sodium fluoride (1g/L) during the demineralizing period served as positive control. After exposure mineral loss and lesion depth of all samples were analysed by transversal microradiography. One-way ANOVA and Student-Newman-Keuls test were used to compare the differences amongst groups. A factorial ANOVA was chosen to test the interaction between GCE and enamel organic matrix.
Mineral loss and lesion depth of specimens in the positive control group were significantly lower compared to all other groups. Regular enamel treated with GCE showed significantly lower values compared to regular enamel treated with DDW or to enamel disposed of its organic matrix (p<0.05). These three groups revealed similar values (p>0.05). Significant interaction between GCE and enamel organic matrix with respect to both outcomes could be observed (p<0.05).
G. chinensis inhibits enamel caries-like demineralization in vitro. However, its potential seems to be weaker compared to sodium fluoride. The organic matrix of enamel was shown to play a substantial role in the observed mechanism.
本体外研究旨在评估五倍子提取物对抑制釉质龋样脱矿的影响，并阐明釉质有机基质在这一过程中的作用。
将牛来源的正常釉质或脱除有机基质的釉质分别暴露于脱矿溶液中 3 天（pH4.5）。另外，将这些标本用 4g/L 的五倍子提取物（GCE）或双蒸水（DDW）每天处理 4 次，每次 5 分钟。在脱矿期间，相应地将正常釉质暴露于氟化钠（1g/L）作为阳性对照。暴露后，通过横切显微放射摄影分析所有样本的矿物损失和病变深度。采用单因素方差分析和 Student-Newman-Keuls 检验比较组间差异。选择析因方差分析来检验 GCE 和釉质有机基质之间的相互作用。
阳性对照组的矿物损失和病变深度明显低于其他所有组。用 GCE 处理的正常釉质与用 DDW 处理的正常釉质或脱除有机基质的釉质相比，其值明显更低（p<0.05）。这三组的数值相似（p>0.05）。可以观察到 GCE 和釉质有机基质在两个结果上存在显著的相互作用（p<0.05）。
五倍子在体外抑制釉质龋样脱矿。然而，其潜力似乎弱于氟化钠。釉质的有机基质在观察到的机制中发挥了重要作用。
