Ceroni M, Camana C, Franciotta D M, Bolelli G, Revello M G, Gerna G, Savoldi F
IRCCS Fondazione C. Mondino Università di Pavia, Sezione di Pavia.
Boll Soc Ital Biol Sper. 1990 Dec;66(12):1223-30.
Total and virus-specific immunoglobulin (Ig) G oligoclonal bands were studied in paired serum and cerebrospinal fluid (CSF) of four patients with herpes simplex virus type 1 (HSV-1) encephalitis. We used the isoelectric focusing in agarose gel, a sensitive technique for protein separation, followed by passive transfer of proteins on nitrocellulose paper and specific immunostaining. Oligoclonal bands were observed in serum and CSF of all patients. HSV-1-specific oligoclonal IgG bands were present in the CSF only during a limited period of the disease, having their counterpart in serum during the remaining periods. Our findings contribute to tackle the issue of B-cell activation within central nervous system and peripheral blood compartments in course of HSV-1 encephalitis.
对4例1型单纯疱疹病毒（HSV-1）脑炎患者的配对血清和脑脊液（CSF）中的总免疫球蛋白（Ig）G和病毒特异性IgG寡克隆带进行了研究。我们采用琼脂糖凝胶等电聚焦技术，这是一种用于蛋白质分离的敏感技术，随后将蛋白质被动转移到硝酸纤维素纸上并进行特异性免疫染色。所有患者的血清和脑脊液中均观察到寡克隆带。HSV-1特异性寡克隆IgG带仅在疾病的有限时期出现在脑脊液中，在其余时期血清中有相应的条带。我们的研究结果有助于解决HSV-1脑炎过程中中枢神经系统和外周血区室中B细胞激活的问题。
