Sköldenberg B, Kalimo K, Carlström A, Forsgren M, Halonen P
Acta Neurol Scand. 1981 May;63(5):273-85.
A solid-phase radioimmunoassay method was used for the detection of herpes simplex virus (HSV) immunoglobulin M (IgM), IgA, and IgG antibodies within the central nervous system in 11 patients with acute HSV encephalitis. Serial cerebrospinal fluid (CSF) and serum specimens were sampled during the observation periods, extending up to 43 months after onset. The clinical diagnosis of HSV encephalitis was confirmed demonstrating virus or virus antigen in the central nervous system in four patients and with significant HSV antibodies in CSF in all the patients. In acute stage CSF HSV antibodies of a significant level were demonstrated in one of four samples taken on days 3--4 after onset, and in samples taken on days 6--8 in five of nine patients. CSF HSV antibodies of a significant and high level were detected in all samples taken from day 10 after onset. Intrathecal production of HSV IgM and IgA antibodies lasted from 7 weeks to 43 months during the observation periods. All patients had persistent intrathecal production of HSV IgG antibodies as well as of oligoclonal IgG during the total observation periods up to 43 months.
采用固相放射免疫分析法检测了11例急性单纯疱疹病毒性脑炎患者中枢神经系统中的单纯疱疹病毒（HSV）免疫球蛋白M（IgM）、IgA和IgG抗体。在长达发病后43个月的观察期内采集系列脑脊液（CSF）和血清标本。4例患者在中枢神经系统中证实存在病毒或病毒抗原，所有患者脑脊液中均有显著的HSV抗体，从而确诊为HSV脑炎。在发病后第3 - 4天采集的4份样本中的1份以及发病后第6 - 8天采集样本的9例患者中的5例，在急性期脑脊液中检测到显著水平的HSV抗体。发病后第10天起采集的所有样本中均检测到显著且高水平的脑脊液HSV抗体。在观察期内，鞘内产生HSV IgM和IgA抗体持续7周至43个月。在长达43个月的整个观察期内，所有患者鞘内持续产生HSV IgG抗体以及寡克隆IgG。
