Kim Young-tae, Karthikeyan Kailash, Chirvi Sajal, Davé Digant P
Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
Lab Chip. 2009 Sep 7;9(17):2576-81. doi: 10.1039/b903720a. Epub 2009 Jul 14.
We describe a neuro-optical microfluidic platform for studying injury and subsequent regeneration of individual mammalian axons. This platform consists of three components integrated on an inverted microscope, which include a compartmentalized neuronal culture microfluidic device, a femtosecond laser to enable precise axotomy, and a custom built mini cell culture incubator for continuous long term observation of post injury events. We demonstrate the unique capabilities of the platform by injuring individual central and peripheral nervous system axons and monitoring the post injury sequence of events from initial degeneration to subsequent regeneration. This platform will enable study and understanding of neuronal response to injury that is currently not possible with conventional cell culture platform and tools.
我们描述了一种用于研究单个哺乳动物轴突损伤及后续再生的神经光学微流控平台。该平台由集成在倒置显微镜上的三个组件组成，包括一个分隔式神经元培养微流控装置、一台用于实现精确轴突切断的飞秒激光以及一个定制的小型细胞培养孵箱，用于对损伤后事件进行连续长期观察。我们通过损伤单个中枢和外周神经系统轴突，并监测从初始退变到后续再生的损伤后事件序列，展示了该平台的独特能力。这个平台将能够研究和理解目前传统细胞培养平台和工具无法实现的神经元对损伤的反应。
