分枝杆菌巯基葡萄糖的生物合成酶的结构和机制。
Structures and mechanisms of the mycothiol biosynthetic enzymes.
机构信息
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
Curr Opin Chem Biol. 2009 Oct;13(4):451-9. doi: 10.1016/j.cbpa.2009.07.018. Epub 2009 Aug 19.
Abstract
In the past decade, the genes encoding all four enzymes responsible for the biosynthesis of mycothiol in Mycobacterium tuberculosis have been identified. Orthologs of each of these have been stably expressed and structurally characterized. The chemical mechanisms of all the four have also been studied. Because of the unique phylogenetic distribution of mycothiol, and the enzymes responsible for its biosynthesis, these enzymes represent interesting potential targets for antimycobacterial agents.
摘要
在过去的十年中，编码分枝杆菌属分枝杆菌生物合成菌巯基葡萄糖的所有四种酶的基因已被鉴定出来。这些酶的同源物已被稳定表达并进行了结构特征分析。四种酶的化学机制也已被研究。由于菌巯基葡萄糖及其生物合成酶独特的系统发育分布，这些酶代表了抗分枝杆菌药物的有趣的潜在靶标。
相似文献
1
Structures and mechanisms of the mycothiol biosynthetic enzymes.分枝杆菌巯基葡萄糖的生物合成酶的结构和机制。
Curr Opin Chem Biol. 2009 Oct;13(4):451-9. doi: 10.1016/j.cbpa.2009.07.018. Epub 2009 Aug 19.
2
Mycothiol-dependent proteins in actinomycetes.放线菌中依赖巯基乙醇辅酶A的蛋白质。
FEMS Microbiol Rev. 2007 Apr;31(3):278-92. doi: 10.1111/j.1574-6976.2006.00062.x. Epub 2007 Feb 26.
3
Synthesis of natural product-inspired inhibitors of Mycobacterium tuberculosis mycothiol-associated enzymes: the first inhibitors of GlcNAc-Ins deacetylase.结核分枝杆菌硫醇相关酶的天然产物启发型抑制剂的合成：N-乙酰葡糖胺-肌醇脱乙酰酶的首批抑制剂
J Med Chem. 2007 Dec 13;50(25):6326-36. doi: 10.1021/jm070669h. Epub 2007 Nov 17.
4
N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis.N-乙酰基-1-D-肌醇-2-氨基-2-脱氧-α-D-吡喃葡萄糖苷脱乙酰酶（MshB）是分枝硫醇生物合成中的关键酶。
J Bacteriol. 2000 Dec;182(24):6958-63. doi: 10.1128/JB.182.24.6958-6963.2000.
5
Synthesis of substrate analogues as potential inhibitors for Mycobacterium tuberculosis enzyme MshC.合成作为结核分枝杆菌MshC酶潜在抑制剂的底物类似物。
Carbohydr Res. 2017 Dec 1;453-454:10-18. doi: 10.1016/j.carres.2017.10.014. Epub 2017 Nov 3.
6
Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis.结核分枝杆菌中一种依赖肌醇硫酯的硝基还原酶的鉴定
ACS Infect Dis. 2018 May 11;4(5):771-787. doi: 10.1021/acsinfecdis.7b00111. Epub 2018 Feb 21.
7
The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases.DinB 超家族包括新型的麦硫因、芽孢硫醇和谷胱甘肽 S-转移酶。
Biochemistry. 2011 Dec 13;50(49):10751-60. doi: 10.1021/bi201460j. Epub 2011 Nov 17.
8
Dequalinium, a new inhibitor of Mycobacterium tuberculosis mycothiol ligase identified by high-throughput screening.地喹氯铵，一种通过高通量筛选鉴定出的新型结核分枝杆菌硫醇连接酶抑制剂。
J Biomol Screen. 2009 Jul;14(6):643-52. doi: 10.1177/1087057109335743. Epub 2009 Jun 12.
9
Mycothiol/mycoredoxin 1-dependent reduction of the peroxiredoxin AhpE from Mycobacterium tuberculosis.分枝杆菌 AhpE 过氧化物酶依赖于分枝菌硫醇/硫氧还蛋白 1 的还原。
J Biol Chem. 2014 Feb 21;289(8):5228-39. doi: 10.1074/jbc.M113.510248. Epub 2013 Dec 30.
10
The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA).编码催化麦角硫因生物合成第一步的酶的糖基转移酶基因（mshA）。
J Bacteriol. 2003 Jun;185(11):3476-9. doi: 10.1128/JB.185.11.3476-3479.2003.
引用本文的文献
1
The mycothiol -transferase is divalent metal-dependent for mycothiol binding and transfer.巯基乙醇转移酶对巯基乙醇的结合和转移依赖二价金属。
RSC Med Chem. 2023 Jan 26;14(3):491-500. doi: 10.1039/d2md00401a. eCollection 2023 Mar 22.
2
Ligand-Induced Conformational and Dynamical Changes in a GT-B Glycosyltransferase: Molecular Dynamics Simulations of Heptosyltransferase I Complexes.配体诱导 GT-B 糖苷转移酶构象和动力学变化：七糖基转移酶 I 复合物的分子动力学模拟。
J Chem Inf Model. 2022 Jan 24;62(2):324-339. doi: 10.1021/acs.jcim.1c00868. Epub 2021 Dec 30.
3
Vaccination Strategies Against Mycobacterium tuberculosis: BCG and Beyond.结核分枝杆菌疫苗接种策略：卡介苗及其他。
Adv Exp Med Biol. 2021;1313:217-240. doi: 10.1007/978-3-030-67452-6_10.
4
Microorganisms: A Potential Source of Bioactive Molecules for Antioxidant Applications.微生物：抗氧化应用中生物活性分子的潜在来源。
Molecules. 2021 Feb 20;26(4):1142. doi: 10.3390/molecules26041142.
5
Small-Molecule Acetylation by GCN5-Related -Acetyltransferases in Bacteria.细菌中 GCN5 相关乙酰转移酶的小分子乙酰化作用。
Microbiol Mol Biol Rev. 2020 Apr 15;84(2). doi: 10.1128/MMBR.00090-19. Print 2020 May 20.
6
Targeting adenylate-forming enzymes with designed sulfonyladenosine inhibitors.针对含有合成磺酰基腺苷抑制剂的腺苷酸形成酶。
J Antibiot (Tokyo). 2019 Jun;72(6):325-349. doi: 10.1038/s41429-019-0171-2. Epub 2019 Apr 15.
7
Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).GCN5相关N-乙酰基转移酶（GNAT）的结构与功能多样性
Int J Mol Sci. 2016 Jun 28;17(7):1018. doi: 10.3390/ijms17071018.
8
Bacterial GCN5-Related N-Acetyltransferases: From Resistance to Regulation.细菌中与GCN5相关的N-乙酰转移酶：从耐药性到调控作用
Biochemistry. 2016 Feb 23;55(7):989-1002. doi: 10.1021/acs.biochem.5b01269. Epub 2016 Feb 9.
9
Metabolic coupling of two small-molecule thiols programs the biosynthesis of lincomycin A.两种小分子硫醇的代谢偶联调控林可霉素 A 的生物合成。
Nature. 2015 Feb 5;518(7537):115-9. doi: 10.1038/nature14137. Epub 2015 Jan 14.
10
X-ray crystallographic structure of BshC, a unique enzyme involved in bacillithiol biosynthesis.BshC的X射线晶体结构，BshC是一种参与杆菌硫醇生物合成的独特酶。
Biochemistry. 2015 Jan 20;54(2):100-3. doi: 10.1021/bi501394q. Epub 2014 Dec 18.
本文引用的文献
1
The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway.耻垢分枝杆菌MshC的1.6埃晶体结构：硫醇生物合成途径中的倒数第二个酶。
Biochemistry. 2008 Dec 16;47(50):13326-35. doi: 10.1021/bi801708f.
2
Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis.巯基乙醇生物合成对于结核分枝杆菌对乙硫异烟胺的敏感性至关重要。
Mol Microbiol. 2008 Sep;69(5):1316-29. doi: 10.1111/j.1365-2958.2008.06365.x. Epub 2008 Jul 21.
3
Glycosyltransferases: structures, functions, and mechanisms.糖基转移酶：结构、功能及作用机制
Annu Rev Biochem. 2008;77:521-55. doi: 10.1146/annurev.biochem.76.061005.092322.
4
Mycothiol regulates and is regulated by a thiol-specific antisigma factor RsrA and sigma(R) in Streptomyces coelicolor.在天蓝色链霉菌中，麦角硫因受硫醇特异性抗西格玛因子RsrA和西格玛（R）调节，并对其产生调控作用。
Mol Microbiol. 2008 May;68(4):861-70. doi: 10.1111/j.1365-2958.2008.06191.x.
5
Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis.谷氨酸棒杆菌MshA的结构与酶学分析：底物辅助催化
J Biol Chem. 2008 Jun 6;283(23):15834-44. doi: 10.1074/jbc.M801017200. Epub 2008 Apr 4.
6
Positional isotope exchange analysis of the Mycobacterium smegmatis cysteine ligase (MshC).耻垢分枝杆菌半胱氨酸连接酶（MshC）的位置同位素交换分析
Biochemistry. 2008 Apr 22;47(16):4843-50. doi: 10.1021/bi800327u. Epub 2008 Mar 29.
7
Synthesis of natural product-inspired inhibitors of Mycobacterium tuberculosis mycothiol-associated enzymes: the first inhibitors of GlcNAc-Ins deacetylase.结核分枝杆菌硫醇相关酶的天然产物启发型抑制剂的合成：N-乙酰葡糖胺-肌醇脱乙酰酶的首批抑制剂
J Med Chem. 2007 Dec 13;50(25):6326-36. doi: 10.1021/jm070669h. Epub 2007 Nov 17.
8
Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC).耻垢分枝杆菌半胱氨酸连接酶（MshC）的稳态和前稳态动力学分析。
Biochemistry. 2007 Oct 9;46(40):11421-9. doi: 10.1021/bi7011492. Epub 2007 Sep 12.
9
Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4.通过来自GT4家族的两种保留型糖基转移酶的结构深入了解脂多糖和抗生素的合成
Chem Biol. 2006 Nov;13(11):1143-52. doi: 10.1016/j.chembiol.2006.09.005.
10
The mshA gene encoding the glycosyltransferase of mycothiol biosynthesis is essential in Mycobacterium tuberculosis Erdman.编码分枝硫醇生物合成中糖基转移酶的mshA基因在结核分枝杆菌埃尔德曼菌株中是必需的。
FEMS Microbiol Lett. 2006 Nov;264(1):74-9. doi: 10.1111/j.1574-6968.2006.00441.x.
Zotero 插件