Baek Jae Ho, Krasieva Tatiana, Tang Shuo, Ahn Yehchan, Kim Chang Soo, Vu Diana, Chen Zhongping, Wilder-Smith Petra
Ulsan University Hospital, Department of Orthodontics, 290-3 Jeonha dong, Dong gu, Ulsan, 682-714, Korea.
J Biomed Opt. 2009 Jul-Aug;14(4):044001. doi: 10.1117/1.3158994.
The salivary pellicle plays an important role in oral physiology, yet noninvasive in situ characterization and mapping of this layer remains elusive. The goal of this study is to develop an optical approach for the real-time, noninvasive mapping and characterization of salivary pellicles using optical coherence tomography (OCT) and optical coherence microscopy (OCM). The long-term goals are to improve diagnostic capabilities in the oral cavity, gain a better understanding of physiological and pathological processes related to the oral hard tissues, and monitor treatment responses. A salivary pellicle is incubated on small enamel cubes using human whole saliva. OCT and OCM imaging occurs at 0, 10, 30, 60 min, and 24 h. For some imaging, spherical gold nanoparticles (15 nm) are added to determine whether this would increase the optical signal from the pellicle. Multiphoton microscopy (MPM) provides the baseline information. In the saliva-incubated samples, a surface signal from the developing pellicle is visible in OCT images. Pellicle "islands" form, which increase in complexity over time until they merge to form a continuous layer over the enamel surface. Noninvasive, in situ time-based pellicle formation on the enamel surface is visualized and characterized using optical imaging.
唾液薄膜在口腔生理学中起着重要作用，然而，对这一层进行非侵入性原位表征和绘图仍然难以实现。本研究的目标是开发一种光学方法，利用光学相干断层扫描（OCT）和光学相干显微镜（OCM）对唾液薄膜进行实时、非侵入性绘图和表征。长期目标是提高口腔诊断能力，更好地了解与口腔硬组织相关的生理和病理过程，并监测治疗反应。使用人全唾液在小的牙釉质立方体上孵育唾液薄膜。OCT和OCM成像分别在0、10、30、60分钟和24小时进行。对于一些成像，添加了球形金纳米颗粒（15纳米）以确定这是否会增加来自薄膜的光学信号。多光子显微镜（MPM）提供基线信息。在唾液孵育的样本中，在OCT图像中可见正在形成的薄膜的表面信号。薄膜“岛”形成，其复杂性随时间增加，直到它们合并形成牙釉质表面上的连续层。使用光学成像对牙釉质表面基于时间的非侵入性原位薄膜形成进行可视化和表征。
