Zamora Perea Elvira, Balta León Rosario, Palomino Salcedo Miriam, Brogdon William G, Devine Gregor J
Laboratorio de Salud Pública, Iquitos, Perú.
Malar J. 2009 Sep 3;8:208. doi: 10.1186/1475-2875-8-208.
The purpose of this study was to establish whether the "bottle assay", a tool for monitoring insecticide resistance in mosquitoes, can complement and augment the capabilities of the established WHO assay, particularly in resource-poor, logistically challenging environments.
Laboratory reared Aedes aegypti and field collected Anopheles darlingi and Anopheles albimanus were used to assess the suitability of locally sourced solvents and formulated insecticides for use with the bottle assay. Using these adapted protocols, the ability of the bottle assay and the WHO assay to discriminate between deltamethrin-resistant Anopheles albimanus populations was compared. The diagnostic dose of deltamethrin that would identify resistance in currently susceptible populations of An. darlingi and Ae. aegypti was defined. The robustness of the bottle assay during a surveillance exercise in the Amazon was assessed.
The bottle assay (using technical or formulated material) and the WHO assay were equally able to differentiate deltamethrin-resistant and susceptible An. albimanus populations. A diagnostic dose of 10 microg a.i./bottle was identified as the most sensitive discriminating dose for characterizing resistance in An. darlingi and Ae. aegypti. Treated bottles, prepared using locally sourced solvents and insecticide formulations, can be stored for > 14 days and used three times. Bottles can be stored and transported under local conditions and field-assays can be completed in a single evening.
The flexible and portable nature of the bottle assay and the ready availability of its components make it a potentially robust and useful tool for monitoring insecticide resistance and efficacy in remote areas that require minimal cost tools.
本研究的目的是确定“瓶试法”（一种监测蚊虫抗药性的工具）是否能够补充并增强既定的世卫组织检测方法的能力，尤其是在资源匮乏、后勤保障具有挑战性的环境中。
使用实验室饲养的埃及伊蚊以及野外采集的达林按蚊和白纹按蚊，评估本地采购的溶剂和配制杀虫剂用于瓶试法的适用性。采用这些经过调整的方案，比较瓶试法和世卫组织检测方法区分对溴氰菊酯具有抗性的白纹按蚊种群的能力。确定了能够鉴别当前敏感的达林按蚊和埃及伊蚊种群中抗性的溴氰菊酯诊断剂量。评估了瓶试法在亚马逊地区一次监测活动中的稳健性。
瓶试法（使用原药或制剂）和世卫组织检测方法同样能够区分对溴氰菊酯具有抗性和敏感的白纹按蚊种群。确定10微克有效成分/瓶的诊断剂量是表征达林按蚊和埃及伊蚊抗性的最敏感鉴别剂量。使用本地采购的溶剂和杀虫剂制剂制备的处理过的瓶子可储存超过14天并使用三次。瓶子可在当地条件下储存和运输，野外检测可在一个晚上完成。
瓶试法灵活便携，其组件易于获取，这使其成为监测偏远地区抗药性和药效的潜在强大且有用的工具，这些地区需要低成本工具。
