Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore 575 018, Karnataka, India.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1765-1769. doi: 10.1099/ijs.0.016840-0. Epub 2009 Sep 11.
A bacterial strain (CC-VM-7T), isolated from the faeces of the pill millipede Arthrosphaera magna Attems collected in India, was studied to determine its taxonomic allocation. Cells stained Gram-negative and were rod-shaped. Comparative analyses of the 16S rRNA gene sequence of the strain with those of the most closely related species clearly suggested allocation to the genus Chryseobacterium, with the highest sequence similarities of 99.2% to Chryseobacterium gleum CCUG 14555T, 98.6% to Chryseobacterium indologenes CCUG 14556T and 98.4% to Chryseobacterium aquifrigidense KCTC 12894T. 16S rRNA gene sequence similarities to all other species of the genus Chryseobacterium were below 98%. The major whole-cell fatty acids were iso-C15:0 and iso-C17:1omega9c. DNA-DNA hybridization resulted in relatedness values of only 29.6% (reciprocal 31.3%) to Chryseobacterium gleum CCUG 14555T, 41.2% (reciprocal 38.8%) to C. indologenes CCUG 14556T and 35.4% (reciprocal 38.5%) to C. aquifrigidense KCTC 12894T. DNA-DNA relatedness, biochemical and chemotaxonomic properties clearly show that strain CC-VM-7T represents a novel species, for which the name Chryseobacterium arthrosphaerae sp. nov. is proposed. The type strain is CC-VM-7T (=CCUG 57618T =CCM 7645T).
一株从印度采集的 Pill 千足虫 Arthrosphaera magna Attems 的粪便中分离出的细菌菌株（CC-VM-7T），被研究以确定其分类归属。细胞经革兰氏染色呈阴性，呈杆状。该菌株的 16S rRNA 基因序列与最密切相关的种的比较分析清楚地表明，它被分配到黄杆菌属，与 Chryseobacterium gleum CCUG 14555T 的序列相似度最高为 99.2%，与 Chryseobacterium indologenes CCUG 14556T 的序列相似度为 98.6%，与 Chryseobacterium aquifrigidense KCTC 12894T 的序列相似度为 98.4%。与黄杆菌属的所有其他种的 16S rRNA 基因序列相似度均低于 98%。主要的全细胞脂肪酸为 iso-C15:0 和 iso-C17:1omega9c。DNA-DNA 杂交的相关性值仅为 29.6%（相互的为 31.3%）与 Chryseobacterium gleum CCUG 14555T，41.2%（相互的为 38.8%）与 Chryseobacterium indologenes CCUG 14556T 和 35.4%（相互的为 38.5%）与 Chryseobacterium aquifrigidense KCTC 12894T。DNA-DNA 相关性、生化和化学分类特征清楚地表明，菌株 CC-VM-7T 代表一个新种，建议将其命名为 Arthrosphaera 黄杆菌（Chryseobacterium arthrosphaerae）sp. nov。模式菌株为 CC-VM-7T（=CCUG 57618T =CCM 7645T）。
