Pialoux Vincent, Brown Allison D, Leigh Richard, Friedenreich Christine M, Poulin Marc J
Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Hypertension. 2009 Nov;54(5):1014-20. doi: 10.1161/HYPERTENSIONAHA.109.138917. Epub 2009 Sep 28.
Increasing evidence exists suggesting an important role for oxidative stress in the pathogenesis and progression of hypertension in women via a decrease of NO production after menopause. Regular physical training has been shown to upregulate antioxidant enzymatic systems, which may slow down the usual increase of oxidative stress in postmenopausal women. The aims of this study were to determine the impact of fitness status on enzymatic antioxidant efficiency, oxidative stress, and NO production and to determine the associations among oxidative stress, enzymatic antioxidant and NO production, mean arterial blood pressure (MABP), and cerebrovascular conductance (CVC) in postmenopausal women (n=40; 50 to 90 years old). Physical fitness, physical activity, resting MABP, and CVC were measured. End product of NO, lipid peroxidation (malondialdehyde and 8-iso-prostaglandin F2alpha), DNA oxidation (8-hydroxy-2'-deoxyguanosine), protein nitration (nitrotyrosine), antioxidant glutathione peroxidase, and catalase activities were measured in plasma. We identified significant negative associations between oxidative stress and indices of physical fitness (malondialdehyde: r=-0.33, P<0.05; 8-iso-prostaglandin F2alpha: r=-0.39, P<0.05; 8-hydroxy-2'-deoxyguanosine: r=-0.35, P<0.05) and physical activity (malondialdehyde: r=-0.30, P<0.05; 8-iso-prostaglandin F2alpha: r=-0.41, P<0.01; 8-hydroxy-2'-deoxyguanosine: r=-0.39, P<0.05). Conversely, glutathione peroxidase was positively correlated with fitness level (r=0.55; P<0.01). Finally, MABP and CVC were significantly associated with 8-hydroxy-2'-deoxyguanosine (MABP: r=0.36, P<0.05; CVC: r=-0.36, P<0.05), nitrotyrosine (MABP: r=0.39, P<0.05; CVC: r=-0.32, P<0.05), and the end product of NO (MABP: r=-0.57, P<0.01; CVC: r=0.44, P<0.01). These findings demonstrate that, after menopause, fitness level and regular physical activity mediate against oxidative stress by maintaining antioxidant enzyme efficiency. Furthermore, these results suggest that oxidative stress and NO production modulate MABP and CVC.
越来越多的证据表明，氧化应激在绝经后女性高血压的发病机制和进展中起着重要作用，其途径是绝经后一氧化氮（NO）生成减少。有研究表明，规律的体育锻炼可上调抗氧化酶系统，这可能会减缓绝经后女性氧化应激的正常增加。本研究的目的是确定健康状况对酶抗氧化效率、氧化应激和NO生成的影响，并确定绝经后女性（n = 40；50至90岁）氧化应激、酶抗氧化剂与NO生成、平均动脉血压（MABP）和脑血管传导（CVC）之间的关联。测量了身体素质、身体活动、静息MABP和CVC。检测了血浆中NO的终产物、脂质过氧化（丙二醛和8-异前列腺素F2α）、DNA氧化（8-羟基-2'-脱氧鸟苷）、蛋白质硝化（硝基酪氨酸）、抗氧化谷胱甘肽过氧化物酶和过氧化氢酶活性。我们发现氧化应激与身体素质指标（丙二醛：r = -0.33，P < 0.05；8-异前列腺素F2α：r = -0.39，P < 0.05；8-羟基-2'-脱氧鸟苷：r = -0.35，P < 0.05）和身体活动（丙二醛：r = -0.30，P < 0.05；8-异前列腺素F2α：r = -0.41，P < 0.01；8-羟基-2'-脱氧鸟苷：r = -0.39，P < 0.05）之间存在显著负相关。相反，谷胱甘肽过氧化物酶与健康水平呈正相关（r = 0.55；P < 0.01）。最后，MABP和CVC与8-羟基-2'-脱氧鸟苷（MABP：r = 0.36，P < 0.05；CVC：r = -0.36，P < 0.05）、硝基酪氨酸（MABP：r = 0.39，P < 0.05；CVC：r = -0.32，P < 0.05）以及NO的终产物（MABP：r = -0.57，P <
