Jiangsu key Laboratory for TCM Formulae Research, College of Pharmacy, Nanjing University of Traditional Chinese Medicine, 210046 Nanjing, China.
Phytochem Anal. 2010 Mar-Apr;21(2):144-9. doi: 10.1002/pca.1170.
The aerial part Eupatorium lindleyanum is commonly used as an antipyretic and detoxicant clinically in traditional Chinese medicine. Our previous research showed that germacrane sesquiterpene lactones were its main active constituents, so the development of rapid and accurate methods for the identification of the sesquiterpene lactones is of great significance.
To develop an HPLC-PDA-ESI-MS/MS method capable for simple and rapid analysis of germacrane sesquiterpene lactones in the aerial part E. lindleyanum.
High-performance liquid chromatography-photodiode array detection-electrospray ionization-tandem mass spectrometry was used to analyze germacrane sesquiterpene lactones of Eupatorium lindleyanum. The fragmentation behavior of germacrane sesquiterpene lactones in a Micromass Q/TOF Mass Spectrometer was discussed, and 9 germacrane sesquiterpene lactones were identified by comparison of their characteristic data of HPLC and MS analyses with those obtained from reference compounds.
The investigated germacrane sesquiterpene lactones were identified as eupalinolides C (1), 3beta-acetoxy-8beta-(4'-hydroxy-tigloyloxy)-14-hydroxy-costunolide (2), eupalinolides A (3), eupalinolides B (4), eupalinolides E (5), 3beta-acetoxy-8beta-(4'-oxo-tigloyloxy)-14-hydroxy-heliangolide (6), 3beta-acetoxy-8beta-(4'-oxo- tigloyloxy)-14-hydroxy-costunolide (7), hiyodorilactone B (8), and 3beta-acetoxy-8beta-(4'-hydroxy-tigloyloxy)- costunolide (9). Compounds 6, 7 and 9 were reported for the first time.
HPLC-PDA-ESI-MS/MS provides a new powerful approach to identify germacrane sesquiterpene lactones in E. lindleyanum rapidly and accurately.
在传统中药中，地上部分艾纳香被广泛用作解热解毒剂。我们之前的研究表明，大牻牛儿苗型倍半萜内酯是其主要活性成分，因此开发快速准确的倍半萜内酯鉴定方法具有重要意义。
建立一种高效液相色谱-光电二极管阵列检测-电喷雾电离串联质谱法，用于简单快速分析艾纳香地上部分的大牻牛儿苗型倍半萜内酯。
采用高效液相色谱-光电二极管阵列检测-电喷雾电离串联质谱法分析艾纳香地上部分的大牻牛儿苗型倍半萜内酯。讨论了大牻牛儿苗型倍半萜内酯在 Micromass Q/TOF 质谱仪中的裂解行为，并通过比较其 HPLC 和 MS 分析的特征数据与参考化合物的特征数据，鉴定了 9 种大牻牛儿苗型倍半萜内酯。
研究的大牻牛儿苗型倍半萜内酯被鉴定为 eupalinolides C（1）、3β-乙酰氧基-8β-（4'-羟基-对伞花烃酰氧基）-14-羟基-古蓬诺内酯（2）、eupalinolides A（3）、eupalinolides B（4）、eupalinolides E（5）、3β-乙酰氧基-8β-（4'-氧代-对伞花烃酰氧基）-14-羟基-贺兰山诺内酯（6）、3β-乙酰氧基-8β-（4'-氧代-对伞花烃酰氧基）-14-羟基-古蓬诺内酯（7）、hiyodorilactone B（8）和 3β-乙酰氧基-8β-（4'-羟基-对伞花烃酰氧基）-古蓬诺内酯（9）。化合物 6、7 和 9 为首次报道。
HPLC-PDA-ESI-MS/MS 为快速准确地鉴定艾纳香中的大牻牛儿苗型倍半萜内酯提供了一种新的有力方法。
