Serviddio G, Romano A D, Greco A, Rollo T, Bellanti F, Altomare E, Vendemiale G
Department of Medical and Occupational Sciences, University of Foggia, IRCCS Casa Sollievo della Sofferenza Hospital, Foggia, Italy.
Int J Immunopathol Pharmacol. 2009 Jul-Sep;22(3):819-27. doi: 10.1177/039463200902200328.
Frailty syndrome (FS) is a condition described in aging and characterized by physical vulnerability to stress and lack of physiological reserve. In this study we aim to define whether circulating oxidative stress correlates to frailty in terms of glutathione balance and oxidative protein damage. In 62 elderly outpatients, classified as frail patients according to Fried's criteria, evaluation of reduced glutathione (GSH), oxidized glutathione (GSSG), tumor necrosis factor-alpha, malonaldehyde-(MDA) and 4-hydroxy-2,3-nonenal-(HNE) protein plasma adducts were performed. A significant increase in the GSSG was observed in patients with FS when compared to non-frail. No difference was shown in the GSH amount, suggesting a glutathione oxidation more than impairment of the synthesis. TNF-alpha, MDA- and HNE-adducts, were significantly higher in FS as compared to non-frail patients. A logistic regression model correlating FS with redox balance showed a close relationship between glutathione ratio (OR=1.8, 95% CI=1.2-2.5) and MDA adducts (OR=2.8, 95% CI=1.6-4.7) to frailty. Our findings show an association between oxidative imbalance and Frailty Syndrome. GSSG/GSH ratio and plasma protein adducts strongly predict the frailty conditions and seem to be reliable and easily measurable markers in the context of the multidimensional analysis of elderly patients.
衰弱综合征（FS）是一种在衰老过程中出现的状况，其特征是身体对应激的易损性以及生理储备的缺乏。在本研究中，我们旨在确定循环氧化应激在谷胱甘肽平衡和氧化蛋白损伤方面是否与衰弱相关。对62名老年门诊患者进行了研究，这些患者根据弗里德标准被分类为衰弱患者，对其血浆中还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、肿瘤坏死因子-α、丙二醛（MDA）和4-羟基-2,3-壬烯醛（HNE）蛋白加合物进行了评估。与非衰弱患者相比，FS患者的GSSG显著增加。GSH含量未显示差异，表明谷胱甘肽氧化程度高于合成受损程度。与非衰弱患者相比，FS患者的TNF-α、MDA和HNE加合物显著更高。将FS与氧化还原平衡相关联的逻辑回归模型显示，谷胱甘肽比率（OR = 1.8，95% CI = 1.2 - 2.5）和MDA加合物（OR = 2.8，95% CI = 1.6 - 4.7）与衰弱密切相关。我们的研究结果表明氧化失衡与衰弱综合征之间存在关联。GSSG/GSH比率和血浆蛋白加合物强烈预测衰弱状况，并且在老年患者的多维分析背景下似乎是可靠且易于测量的标志物。
