Niklasson B, Tkachenko E, Ivanov A P, van der Groen G, Wiger D, Andersen H K, LeDuc J, Kjelsson T, Nyström K
National Bacteriological Laboratory, Stockholm, Sweden.
Res Virol. 1990 Nov-Dec;141(6):637-48. doi: 10.1016/0923-2516(90)90036-i.
IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the "one-way" crossing described earlier.
使用来自不同地理区域（瑞典、丹麦、挪威、比利时和苏联）的肾综合征出血热（HFRS）患者的血清，对普马拉病毒的IgM和IgG酶联免疫吸附测定（ELISA）进行了评估。IgM ELISA被证明对上述所有地区患者的HFRS诊断有用。特异性IgM最早可在发病后第1天检测到，患者在数月内一直呈IgM阳性。在急性期血清中也经常检测到特异性IgG ELISA抗体，急性期与恢复期血清配对检测时，往往未显示出显著的滴度升高或光密度（OD）值增加。这限制了IgG ELISA在患者诊断中的应用。感染后2年采集的血清显示，其IgG ELISA OD读数高于恢复期血清，感染后10至20年仍可检测到非常高的值。因此，IgG ELISA可用于免疫检测和血清流行病学研究。韩国和苏联亚洲地区HFRS患者的急性期和恢复期血清在普马拉病毒IgG和IgM ELISA中无反应或反应非常微弱。这些结果与之前描述的“单向”交叉情况一致。
