He Yao, Zhu Huifeng, Li Wanyu, Chen Gang, Li Zhuoheng, Xu Xiaoyu
College of Pharmacy, Chongqing University of Medical Science, Chongqing 400016, China.
Zhongguo Zhong Yao Za Zhi. 2009 Jul;34(13):1717-9.
To establish an HPLC method for determination of catalpol in CSF (cerebrospinal fluid) of rats.
Rats were intravenously injected 1.0 g x L(-1) catalpol physiological saline, and the sample of CSF from subarachnoid space of the cerebrum 40 minutes of injection. The sample of CSF from normal rats was used for blank control, the all samples were preserved in a refrigerator of - 20 degrees C, and use HPLC was employed to determine the catalpol content. The separation of catalpol was performed on Hypersil C18 reversion phase chromatographic column. The mobile phase consisted of water-acetonitrile (99.5: 0.5) with a flow rate of 1.0 mL x min(-1) and detection wavelength of 210 nm.
The linear range of catalpol in CSF was 0.5-40 mg x L(-1) (r = 0.999 7). The absolute recoveries were (90.2 +/- 1.71)%, (89.1 +/- 1.17)% and (86.9 +/- 0.98)%; and the methodological recoveries were (99.8 +/- 1.98)%, (101.1 +/- 3.04)%, (100.1 +/- 2.30)% respectively. The within-day and between-day derivation RSD were less than 4%. Catalpol was stable in a refrigerator of -20 degrees C for 15 days.
The method is simple and accurate for the determination of the content of catalpol in CSF.
建立一种测定大鼠脑脊液（CSF）中梓醇含量的高效液相色谱法。
给大鼠静脉注射1.0 g·L⁻¹梓醇生理盐水，注射40分钟后采集大脑蛛网膜下腔的脑脊液样本。以正常大鼠的脑脊液样本作为空白对照，所有样本均保存在-20℃冰箱中，采用高效液相色谱法测定梓醇含量。梓醇的分离在Hypersil C18反相色谱柱上进行。流动相为水-乙腈（99.5∶0.5），流速为1.0 mL·min⁻¹，检测波长为210 nm。
脑脊液中梓醇的线性范围为0.5～40 mg·L⁻¹（r = 0.999 7）。绝对回收率分别为（90.2±1.71）%、（89.1±1.17）%和（86.9±0.98）%；方法回收率分别为（99.8±1.98）%、（101.1±3.04）%、（100.1±2.30）%。日内和日间精密度RSD均小于4%。梓醇在-20℃冰箱中15天内稳定。
该方法用于测定脑脊液中梓醇含量简便、准确。
