从一株经猿猴病毒40转化的人类细胞系中纯化猿猴病毒40肿瘤抗原。

Tenen D G, Garewal H, Haines L L, Hudson J, Woodard V, Light S, Livingston D M

Proc Natl Acad Sci U S A. 1977 Sep;74(9):3745-9. doi: 10.1073/pnas.74.9.3745.

Simian virus 40 tumor antigen can be isolated in a highly purified state from the nuclei ofSV80 cells, a continuous line of simian virus 40-transformed human fibroblasts. A five-step purification method was used. Its apparent molecular weight (in sodium dodecyl sulfate/polyacrylamide gels) is approximately 90,000-94,000. It contains a detectable amino-terminal residue.

猿猴病毒40肿瘤抗原可从SV80细胞的细胞核中以高度纯化的状态分离出来，SV80细胞是猿猴病毒40转化的人成纤维细胞的连续细胞系。采用了五步纯化方法。其表观分子量（在十二烷基硫酸钠/聚丙烯酰胺凝胶中）约为90,000 - 94,000。它含有一个可检测到的氨基末端残基。