Agricultural Research Organization, Institute of Soil, Water and Environmental Sciences, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel.
J Agric Food Chem. 2009 Dec 9;57(23):11254-60. doi: 10.1021/jf901984k.
Concentrations of dissolved organic carbon (DOC) and total phenols (TP), and the phytotoxicity to cress (Lepidium sativum L.) were determined for three molecular-sized fractions of olive mill wastewater (OMW), <1000, 1000-5000, and >5000 Da, before and after incubation with Pleurotus ostreatus. The <1000-Da fraction contained 82% of the total DOC and 48% of the TP, and was the most phytotoxic. Ethyl acetate separation of aqueous and solvent fractions showed that the aqueous fraction contained 93% of the total DOC, 83% of the TP, and was most phytotoxic, indicating low importance of monomeric phenols. Incubation of whole OMW and of the separate size fractions with P. ostreatus mycelia reduced TP by factors of 4.3-5.3, but exerted diverse impact on phytotoxicity; overall, P. ostreatus efficacy in organic load removal and OMW detoxification was limited. Additional size fractionation of the incubated fractions revealed that most residual phytotoxicity was associated with low-molecular weight (MW) compounds originated from the <1000 Da fraction and not with low-MW byproducts from the degradation of higher-MW fractions and that polymerized metabolites were nonphytotoxic. Total phenols should not be used as sole indicators of the successful remediation of OMW.
测定了橄榄厂废水（OMW）<1000、1000-5000 和>5000 Da 三种分子大小的馏分在与糙皮侧耳（Pleurotus ostreatus）孵育前后的溶解有机碳（DOC）和总酚（TP）浓度，以及对水芹（Lepidium sativum L.）的植物毒性。<1000-Da 馏分含有 82%的总 DOC 和 48%的总 TP，具有最高的植物毒性。水相与溶剂相的乙酸乙酯分离表明，水相含有 93%的总 DOC、83%的总 TP，具有最高的植物毒性，表明低分子量酚类物质的重要性较低。用糙皮侧耳菌丝体孵育整个 OMW 及其单独的大小馏分，可将 TP 减少 4.3-5.3 倍，但对植物毒性的影响不同；总的来说，糙皮侧耳去除有机负荷和 OMW 解毒的功效有限。孵育馏分的进一步分级分离表明，大部分残留的植物毒性与来源于<1000 Da 馏分的低分子量（MW）化合物有关，而不是与降解高分子量馏分产生的低分子量副产物有关，并且聚合代谢物没有植物毒性。总酚类物质不应作为成功修复 OMW 的唯一指标。
