Strle Klemen, Drouin Elise E, Shen Shiqian, El Khoury Joseph, McHugh Gail, Ruzic-Sabljic Eva, Strle Franc, Steere Allen C
Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
J Infect Dis. 2009 Dec 15;200(12):1936-43. doi: 10.1086/648091.
To delineate the inflammatory potential of the 3 pathogenic species of Borrelia burgdorferi sensu lato, we stimulated monocyte-derived macrophages from healthy human donors with 10 isolates each of B. burgdorferi, Borrelia afzelii, or Borrelia garinii recovered from erythema migrans skin lesions of patients with Lyme borreliosis from the United States or Slovenia. B. burgdorferi isolates from the United States induced macrophages to secrete significantly higher levels of interleukin (IL)-8, CCL3, CCL4, IL-6, IL-10, and tumor necrosis factor than B. garinii or B. afzelii isolates. Consistent with this response in cultured macrophages, chemokine and cytokine levels in serum samples of patients from whom the isolates were obtained were significantly greater in B. burgdorferi-infected patients than in B. afzelii- or B. garinii-infected patients. These results demonstrate in vitro and in vivo that B. burgdorferi has greater inflammatory potential than B. afzelii and B. garinii, which may account in part for variations in the clinical manifestations of Lyme borreliosis.
为了阐明狭义伯氏疏螺旋体的3种致病菌种的炎症潜能，我们用从美国或斯洛文尼亚莱姆病患者的游走性红斑皮肤损伤中分离出的10株伯氏疏螺旋体、阿氏疏螺旋体或伽氏疏螺旋体，分别刺激来自健康人类供体的单核细胞衍生巨噬细胞。与伽氏疏螺旋体或阿氏疏螺旋体分离株相比，来自美国的伯氏疏螺旋体分离株诱导巨噬细胞分泌显著更高水平的白细胞介素（IL）-8、CCL3、CCL4、IL-6、IL-10和肿瘤坏死因子。与培养巨噬细胞中的这种反应一致，分离出这些菌株的患者血清样本中的趋化因子和细胞因子水平，在感染伯氏疏螺旋体的患者中显著高于感染阿氏疏螺旋体或伽氏疏螺旋体的患者。这些结果在体外和体内均表明，伯氏疏螺旋体比阿氏疏螺旋体和伽氏疏螺旋体具有更大的炎症潜能，这可能部分解释了莱姆病临床表现的差异。
