Univ Lille Nord de France, F-59000 Lille, France.
Sci Total Environ. 2010 Feb 1;408(5):1166-73. doi: 10.1016/j.scitotenv.2009.10.073. Epub 2009 Nov 17.
To evaluate the utility of urinary 3-hydroxybenzo[a]pyrene (3-OH-B[a]P) as a biomarker of exposure to polycyclic aromatic hydrocarbons (PAHs) in various environmental exposure scenarios alongside the more usually studied 1-hydroxypyrene (1-OH-Pyr).
Two groups of 15 and 10 non smoking, healthy men and women, were exposed for approximately 6h to ambient air at two outdoor locations close to metallurgical industries, and at one indoor location in an urban setting. Atmospheric measurements of 16 "priority" PAHs were carried out during each exposure. Urinary 1-OH-Pyr and 3-OH-B[a]P were also analysed, samples being taken the morning before exposure, at the end of exposure, then 4 and 15 h after the end of exposure. Urinary 1-OH-Pyr and 3-OH-B[a]P were analysed using high performance liquid chromatograph with fluorescence detection. Limits of detection (LOD) were 0.092 nmol/L and 0.28 pmol/L for 1-OH-Pyr and 3-OH-B[a]P respectively.
Average ambient air concentrations varied from 0.27 to 2.87 ng/m(3) for pyrene, 0.04 to 1.20 ng/m(3) for B[a]P, and from 70.0 to 910.6 ng/m(3) for the sum of the 16 PAH (SigmaPAHs). Concentrations of 1-OH-Pyr were not correlated with atmospheric concentrations of PAHs to which subjects were exposed, nor with the concentrations of 3-OH-B[a]P. Nearly 80% of measurements of 3-OH-B[a]P were lower than the LOD and no relationship between atmospheric concentrations and urinary metabolites was observable. However, the percentage of post-exposure values of 3-OH-B[a]P greater than the LOD increased significantly with the median of atmospheric concentrations of Pyr, B[a]P and SigmaPAH at the different sites (test of linear trend, p<0.02 in all cases).
Although we used very sensitive techniques for the measurements of urinary metabolites, especially for 3-OH-B[a]P, neither 1-OH-Pyr nor 3-OH-B[a]P were an unequivocal biomarker of exposure to atmospheric PAHs in environmental exposure scenarios relevant to the general population. It would be interesting to investigate other urinary monohydroxy PAH metabolites in this context.
评估尿液中的 3-羟基苯并[a]芘（3-OH-B[a]P）作为多环芳烃（PAHs）暴露生物标志物的效用，同时研究更常研究的 1-羟基芘（1-OH-Pyr）。
两组 15 名和 10 名非吸烟、健康的男性和女性分别在靠近冶金工业的两个户外地点和城市环境中的一个室内地点暴露约 6 小时。在每次暴露期间进行了 16 种“优先”PAHs 的大气测量。还分析了尿液中的 1-OH-Pyr 和 3-OH-B[a]P，在暴露前的早晨、暴露结束时、暴露结束后 4 小时和 15 小时采集样本。使用带有荧光检测的高效液相色谱法分析尿液中的 1-OH-Pyr 和 3-OH-B[a]P。1-OH-Pyr 和 3-OH-B[a]P 的检测限（LOD）分别为 0.092 nmol/L 和 0.28 pmol/L。
空气中的平均浓度为：苊为 0.27-2.87ng/m3，苯并[a]芘为 0.04-1.20ng/m3，十六种多环芳烃总和（SigmaPAHs）为 70.0-910.6ng/m3。1-OH-Pyr 浓度与暴露于其中的大气 PAHs 浓度无关，也与 3-OH-B[a]P 浓度无关。3-OH-B[a]P 的测量值中有近 80%低于 LOD，并且无法观察到大气浓度与尿代谢物之间的关系。然而，暴露后 3-OH-B[a]P 值大于 LOD 的百分比随着不同地点空气中 Pyr、B[a]P 和 SigmaPAH 的中位数呈显著增加（在所有情况下，线性趋势检验，p<0.02）。
尽管我们使用非常敏感的技术来测量尿液代谢物，特别是 3-OH-B[a]P，但 1-OH-Pyr 和 3-OH-B[a]P 都不是人群环境暴露场景中大气 PAHs 暴露的明确生物标志物。在这种情况下，研究其他尿液单羟基多环芳烃代谢物将很有趣。
