Department of Life Science, College of Agriculture, Tamagawa University, Tokyo, Japan.
Molecules. 2009 Nov 4;14(11):4425-32. doi: 10.3390/molecules14114425.
The aim of the present work was to clarify the anti-melanogenic mechanism of the catechin group. In this study, we used (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-catechin (C), and gallic acid (GA). The catechin group inhibited melanin synthesis in B16 melanoma cells. To elucidate the anti-melanogenic mechanism of the catechin group, we performed Western blotting analysis for crucial melanogenic protein, namely tyrosinase. The catechin group inhibited tyrosinase expression. These results indicate that the catechin group is a candidate anti-melanogenic agent and that it might be effective in hyperpigmentation disorders.
本研究旨在阐明儿茶素族的抗黑色素生成机制。在这项研究中，我们使用了 (-)-表没食子儿茶素-3-没食子酸酯（EGCG）、(-)-表儿茶素（EGC）、(-)-儿茶素（C）和没食子酸（GA）。儿茶素族抑制了 B16 黑色素瘤细胞中的黑色素合成。为了阐明儿茶素族的抗黑色素生成机制，我们对关键的黑色素生成蛋白——酪氨酸酶进行了 Western 印迹分析。儿茶素族抑制了酪氨酸酶的表达。这些结果表明，儿茶素族是一种候选的抗黑色素生成剂，可能对色素沉着异常有效。
