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茶多酚通过抑制α-促黑素细胞激素-黑素皮质素受体1信号通路对紫外线A诱导的黑素生成的影响。

Effects of tea polyphenols on UVA-induced melanogenesis via inhibition of α-MSH-MC1R signalling pathway.

作者信息

Chen Jiaoquan, Li Huaping, Liang Bihua, Zhu Huilan

机构信息

Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou, Guangdong, China.

出版信息

Postepy Dermatol Alergol. 2022 Apr;39(2):327-335. doi: 10.5114/ada.2022.115890. Epub 2022 May 9.

DOI:10.5114/ada.2022.115890
PMID:35645678
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9131962/
Abstract

INTRODUCTION

Ultraviolet (UV) irradiation is a major environmental factor affecting photoaging, which is characterized by skin wrinkle formation and hyperpigmentation. Although many factors are involved in the melanogenesis progress, UV is thought to play a major role in tanning. The pathway of α-melanocyte-stimulating hormone (α-MSH)-melanocortin receptor 1 (MC1R) is associated with UV-induced melanogenesis. Thus, α-MSH antagonists may have applications in the prevention of melanogenesis.

AIM

To investigate the effects of tea polyphenols (TPS) on pigmentation, and further explore the underlying mechanism.

MATERIAL AND METHODS

Human keratinocyte cell line (HaCaT) cells and Human epidermal melanocytes (HEM) were exposed to UVA and treated with different concentrations of TPS or Nonapeptide-1 acetate salt (N-1A). Then, cell viability, melanin content, and tyrosinase activity of both kinds of cells were detected. Quantification of α-MSH in HaCaT cells and HEM cells determined by ELISA assays. Immunohistochemistry of HEM cells was employed to further investigate the expression of melanogenesis-related proteins.

RESULTS

The different concentrations of TPS were found to decrease the melanin content, tyrosinase activity and melanogenesis-related proteins such as microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)1, and TRP2. Besides, TPS inhibited α-MSH-MC1R signalling through directly suppressed α-MSH expression rather than the down-regulated expression level of MC1R.

CONCLUSIONS

Our findings indicate that TPS may be a potential whitening agent for use in cosmetics and the medical treatment of hyperpigmentation disorders.

摘要

引言

紫外线（UV）照射是影响光老化的主要环境因素，其特征为皮肤皱纹形成和色素沉着。尽管黑色素生成过程涉及许多因素，但紫外线被认为在晒黑中起主要作用。α-黑素细胞刺激激素（α-MSH）-黑素皮质素受体1（MC1R）途径与紫外线诱导的黑色素生成有关。因此，α-MSH拮抗剂可能在预防黑色素生成方面有应用。

目的

研究茶多酚（TPS）对色素沉着的影响，并进一步探讨其潜在机制。

材料与方法

将人角质形成细胞系（HaCaT）细胞和人表皮黑素细胞（HEM）暴露于UVA，并分别用不同浓度的TPS或九肽-1醋酸盐（N-1A）处理。然后，检测两种细胞的细胞活力、黑色素含量和酪氨酸酶活性。通过ELISA测定法对HaCaT细胞和HEM细胞中的α-MSH进行定量。采用HEM细胞免疫组织化学法进一步研究黑色素生成相关蛋白的表达。

结果

发现不同浓度的TPS可降低黑色素含量、酪氨酸酶活性以及小眼畸形相关转录因子（MITF）、酪氨酸酶相关蛋白（TRP）1和TRP2等黑色素生成相关蛋白的表达。此外，TPS通过直接抑制α-MSH表达而非下调MC1R的表达水平来抑制α-MSH-MC1R信号通路。

结论

我们的研究结果表明，TPS可能是一种潜在的美白剂，可用于化妆品和色素沉着紊乱的医学治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/26bb9b671f2a/PDIA-39-46951-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/743386c4e48c/PDIA-39-46951-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/035b4ef8f9f7/PDIA-39-46951-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/80128acbb6d6/PDIA-39-46951-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/09a513227898/PDIA-39-46951-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/a4c7fdf84453/PDIA-39-46951-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/26bb9b671f2a/PDIA-39-46951-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/743386c4e48c/PDIA-39-46951-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/035b4ef8f9f7/PDIA-39-46951-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/80128acbb6d6/PDIA-39-46951-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/09a513227898/PDIA-39-46951-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/a4c7fdf84453/PDIA-39-46951-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d58/9131962/26bb9b671f2a/PDIA-39-46951-g006.jpg

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