Murray P R
Barnes Hospital Clinical Microbiology Laboratory, Washington University School of Medicine, Saint Louis, Missouri 63110.
J Clin Microbiol. 1991 Jan;29(1):96-8. doi: 10.1128/jcm.29.1.96-98.1991.
Although the detection of fungemia has been improved by the use of vented or biphasic blood culture bottles, the best recovery and earliest detection have been reported in the Isolator lysis-centrifugation system. It was recently demonstrated that improved detection of both bacteria and fungi was accomplished by mechanically agitating blood culture bottles for the first 24 h of incubation. In this study the detection of fungemia by use of the Isolator system was compared with that of an agitated biphasic system. A total of 182 fungi were isolated from blood specimens inoculated into both culture systems. No difference in the overall recovery of fungi or individual species of yeasts was observed between the two systems. However, all seven isolates of Histoplasma capsulatum were recovered in the Isolator system only. The time required to detect fungemia with each of the two systems was also compared. No statistically significant difference was observed. From the data collected during this 18-month study, it can be concluded that the overall recovery and time of detection of yeasts are equivalent in the lysis-centrifugation system and the agitated biphasic blood culture system. The lysis-centrifugation system is still superior for the detection of filamentous fungi such as H. capsulatum.
尽管使用通气或双相血培养瓶提高了真菌血症的检测率，但据报道，在伊索莱特裂解离心系统中回收率最高且能最早检测到真菌。最近有研究表明，在培养的头24小时对血培养瓶进行机械搅拌可提高细菌和真菌的检测率。在本研究中，将使用伊索莱特系统检测真菌血症的情况与搅拌双相系统进行了比较。从接种到两种培养系统的血液标本中总共分离出182株真菌。两种系统在真菌的总体回收率或酵母的各个种类方面均未观察到差异。然而，所有7株荚膜组织胞浆菌均仅在伊索莱特系统中被分离出来。还比较了两种系统检测真菌血症所需的时间。未观察到统计学上的显著差异。从这项为期18个月的研究收集的数据可以得出结论，在裂解离心系统和搅拌双相血培养系统中，酵母的总体回收率和检测时间相当。裂解离心系统在检测丝状真菌如荚膜组织胞浆菌方面仍然更具优势。
