Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, Germany.
Eur J Endocrinol. 2010 Mar;162(3):507-13. doi: 10.1530/EJE-09-0737. Epub 2009 Dec 1.
To study the association of vaspin with glucose metabolism.
Cross-sectional and intervention study.
The association of serum vaspin with metabolic and anthropometric characteristics was investigated in 108 volunteers. Euglycemic-hyperinsulinemic clamps (EHC) were performed in 83 of the participants. Changes of circulating vaspin levels were additionally studied in a crossover study using 300 min EHC with lipid versus saline infusion (n=10).
Neither glucose tolerance status nor insulin sensitivity, both as measured using EHCs and using homeostasis model assessment for insulin resistance (HOMA-IR), was significantly associated with serum vaspin in the cross-sectional study. Furthermore, there was no effect of short-term lipid-induced insulin resistance due to a 300 min intravenous lipid challenge on circulating vaspin. However, circulating vaspin levels were significantly elevated in women using oral contraceptives (OC), both compared to women without OC intake (1.17+/-0.26 vs 0.52+/-0.09 ng/ml, P=0.02) and males (1.17+/-0.26 vs 0.29+/-0.04 ng/ml, P=0.01). After exclusion of OC using females and stratification according to body mass index (BMI), a significant sexual dimorphism in subjects with a BMI <25 kg/m(2) was observed (males 0.21+/-0.04 ng/ml versus females 0.70+/-0.16 ng/ml, P=0.009).
Our results support the existence of a sexual dimorphism regarding circulating vaspin. The lack of an association of serum vaspin with HOMA-IR and M value indicates, however, no major role for vaspin concerning insulin sensitivity in nondiabetic humans.
研究内脂素与糖代谢的关系。
横断面研究和干预研究。
研究了 108 名志愿者血清内脂素与代谢和人体测量特征的关系。83 名参与者进行了正常血糖高胰岛素钳夹试验（EHC）。在使用脂质与盐水输注的 300 分钟 EHC 的交叉研究中，另外研究了循环内脂素水平的变化（n=10）。
在横断面研究中，无论是使用 EHC 还是使用稳态模型评估胰岛素抵抗（HOMA-IR）测量的葡萄糖耐量状态或胰岛素敏感性，均与血清内脂素无显著相关性。此外，由于 300 分钟静脉内脂质挑战引起的短期脂质诱导的胰岛素抵抗对内脂素无影响。然而，口服避孕药（OC）使用者的循环内脂素水平明显升高，与未服用 OC 者相比（1.17±0.26 与 0.52±0.09ng/ml，P=0.02）和男性（1.17±0.26 与 0.29±0.04ng/ml，P=0.01）。排除 OC 对女性的影响，并根据体重指数（BMI）进行分层后，观察到 BMI<25kg/m2 的受试者存在明显的性别二态性（男性 0.21±0.04ng/ml 与女性 0.70±0.16ng/ml，P=0.009）。
我们的结果支持循环内脂素有性别二态性的存在。然而，血清内脂素与 HOMA-IR 和 M 值无关联表明，内脂素在非糖尿病人群中对胰岛素敏感性无主要作用。
