Kuhn R M, Clarke L, Carbon J
Department of Biological Sciences, University of California, Santa Barbara 93106.
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1306-10. doi: 10.1073/pnas.88.4.1306.
The centromere-associated B' and B DNA sequence repeats of Schizosaccharomyces pombe chromosomes I and II have been found to contain clusters of tRNA genes. The centromere II region (cen2) includes at least 22 tRNA genes distributed among five copies of the B sequence repeat containing genes specifying tRNA(Ile), tRNA(Ala), and tRNA(Val). Individual B repeats are variously associated with other tRNA genes, including those specifying tRNA(Lys), tRNA(Arg), and tRNA(Glu2). The centromere I region (cen1) contains at least six tRNA genes in two copies of the B' repeated element, including genes specifying tRNA(Ile), tRNA(Ala), and tRNA(Glu3). Multiple tandemly arranged clusters of tRNA genes are presumably conserved due to restricted recombination frequencies in the centromere regions.
已发现粟酒裂殖酵母I号和II号染色体上与着丝粒相关的B'和B DNA序列重复序列含有tRNA基因簇。着丝粒II区域（cen2）包括至少22个tRNA基因，分布在B序列重复序列的五个拷贝中，这些拷贝包含指定tRNA（Ile）、tRNA（Ala）和tRNA（Val）的基因。单个B重复序列与其他tRNA基因有不同的关联，包括指定tRNA（Lys）、tRNA（Arg）和tRNA（Glu2）的基因。着丝粒I区域（cen1）在B'重复元件的两个拷贝中包含至少六个tRNA基因，包括指定tRNA（Ile）、tRNA（Ala）和tRNA（Glu3）的基因。由于着丝粒区域的重组频率受限，多个串联排列的tRNA基因簇可能得以保留。
