Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
J Infect Dis. 2010 Jan 15;201(2):264-71. doi: 10.1086/649227.
The therapeutic potential of bacteriophages (phages) in a mouse model of acute Burkholderia cenocepacia pulmonary infection was assessed. Phage treatment was administered by either intranasal inhalation or intraperitoneal injection. Bacterial density, macrophage inflammatory protein 2 (MIP-2), and tumor necrosis factor alpha (TNF-alpha) levels were significantly reduced in lungs of mice treated with intraperitoneal phages (P < .05). No significant differences in lung bacterial density or MIP-2 levels were found between untreated mice and mice treated with intranasal phages, intraperitoneal ultraviolet-inactivated phages, or intraperitoneal lambda phage control mice. Mock-infected mice treated with phage showed no significant increase in lung MIP-2 or TNF-alpha levels compared with mock-infected/mock-treated mice. We have demonstrated the efficacy of phage therapy in an acute B. cenocepacia lung infection model. Systemic phage administration was more effective than inhalational administration, suggesting that circulating phages have better access to bacteria in lungs than do topical phages.
我们评估了噬菌体（phages）在急性洋葱伯克霍尔德氏菌肺部感染小鼠模型中的治疗潜力。噬菌体治疗通过鼻内吸入或腹腔注射进行。腹腔注射噬菌体治疗可显著降低肺部的细菌密度、巨噬细胞炎症蛋白 2（MIP-2）和肿瘤坏死因子-α（TNF-α）水平（P＜0.05）。未治疗的小鼠与鼻内噬菌体、腹腔紫外线灭活噬菌体或腹腔 lambda 噬菌体对照小鼠相比，肺部细菌密度或 MIP-2 水平无显著差异。与未感染/未治疗的小鼠相比，用噬菌体 mock 感染的小鼠肺部 MIP-2 或 TNF-α水平没有显著增加。我们已经证明了噬菌体治疗在急性洋葱伯克霍尔德氏菌肺部感染模型中的疗效。全身噬菌体给药比吸入给药更有效，这表明循环噬菌体比局部噬菌体更容易进入肺部的细菌。
