Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
BMC Med Genet. 2009 Dec 9;10:131. doi: 10.1186/1471-2350-10-131.
Common FTO (fat mass and obesity associated) gene variants have recently been strongly associated with body mass index and obesity in several large studies. Here we set out to examine the association of the FTO variant rs9939609 with BMI in a 32 year follow up study of men born 1920-1924. Moreover, we analyzed the effect of physical activity on the different genotypes.
The FTO rs9936609 was genotyped using an Illumina golden gate assay. BMI was calculated using standard methods and body fat was estimated by measuring skinfold thickness using a Harpenden caliper. Physical activity was assessed using a four question medical questionnaire.
FTO rs9939609 was genotyped in 1153 elderly Swedish men taking part of a population-based cohort study, the ULSAM cohort. The risk of obesity and differences in BMI according to genotype at the ages of 50, 60, 70, 77 and 82 were investigated. We found no increased risk of obesity and no association with BMI at any age with the FTO rs9939609 variant. We found however interaction between physical activity at the age of 50 years and genotype on BMI levels (p = 0.039) and there was a clear trend towards larger BMI differences between the TT and AA carriers as well as between AT and AA carriers in the less physically active subjects.
Here we found that the well established obesity risk allele for a common variant in FTO does not associate with increased BMI levels in a Swedish population of adult men which reached adulthood before the appearance of today's obesogenic enviroment. There is an interaction between physical activity and the effect of the FTO genotype on BMI levels suggesting that lack of physical activity is a requirement for an association of FTO gene variants to obesity.
在几项大型研究中，常见的 FTO（脂肪量和肥胖症相关）基因变体最近与体重指数和肥胖症密切相关。在这里，我们着手研究 FTO 变体 rs9939609 与 1920-1924 年出生的男性 32 年随访研究中 BMI 的关系。此外，我们分析了体力活动对不同基因型的影响。
使用 Illumina 金标检测法对 FTO rs9936609 进行基因分型。BMI 通过标准方法计算，体脂通过使用 Harpenden 卡尺测量皮褶厚度来估算。体力活动通过四个问题的医学问卷进行评估。
在参与基于人群的队列研究 ULSAM 队列的 1153 名瑞典老年男性中，对 FTO rs9939609 进行了基因分型。研究了在 50、60、70、77 和 82 岁时，肥胖风险和基因型对 BMI 的影响。我们没有发现肥胖风险增加，也没有发现 FTO rs9939609 变体与任何年龄的 BMI 相关。但是，我们发现 50 岁时的体力活动与基因型对 BMI 水平存在交互作用（p = 0.039），在体力活动较少的受试者中，TT 和 AA 携带者以及 AT 和 AA 携带者之间的 BMI 差异明显更大。
在这里，我们发现 FTO 中常见变体的已确立肥胖风险等位基因与瑞典成年男性的 BMI 水平增加无关，这些男性在当今肥胖环境出现之前就已成年。体力活动与 FTO 基因型对 BMI 水平的影响之间存在相互作用，这表明缺乏体力活动是 FTO 基因变异与肥胖相关的必要条件。
