Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.
Appl Environ Microbiol. 2010 Feb;76(4):1241-50. doi: 10.1128/AEM.02153-09. Epub 2009 Dec 18.
This paper describes a new approach for labeling intact flagella using the biarsenical dyes FlAsH and ReAsH and imaging their spatial and temporal dynamics on live Escherichia coli cells in swarming communities of bacteria by using epifluorescence microscopy. Using this approach, we observed that (i) bundles of flagella on swarmer cells remain cohesive during frequent collisions with neighboring cells, (ii) flagella on nonmotile swarmer cells at the leading edge of the colony protrude in the direction of the uncolonized agar surface and are actively rotated in a thin layer of fluid that extends outward from the colony, and (iii) flagella form transient interactions with the flagella of other swarmer cells that are in close proximity. This approach opens a window for observing the dynamics of cells in communities that are relevant to ecology, industry, and biomedicine.
本文描述了一种新的方法，用于使用双砷染料 FlAsH 和 ReAsH 对完整的鞭毛进行标记，并通过荧光显微镜观察在 swarm 状态的细菌群体中活大肠杆菌细胞上鞭毛的时空动态。使用这种方法，我们观察到：（i）在与相邻细胞频繁碰撞的过程中， swarm 细胞上的鞭毛束保持凝聚性；（ii）在菌落前沿的非运动 swarm 细胞上的鞭毛向未被殖民的琼脂表面突出，并在从菌落向外延伸的薄层流体中积极旋转；（iii）鞭毛与近距离的其他 swarm 细胞的鞭毛形成短暂的相互作用。这种方法为观察与生态学、工业和生物医学相关的群落中细胞的动态提供了一个窗口。
