Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Int J Obes (Lond). 2010 Mar;34(3):578-88. doi: 10.1038/ijo.2009.266. Epub 2009 Dec 22.
To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD).
Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis.
Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming.
Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.
分析两种甜橙（Citrus sinensis L. Osbeck）果汁，即血橙（Moro）和脐橙（Navelina）对标准饮食或高脂饮食（HFD）喂养的小鼠脂肪积累的影响。
通过喂养 HFD 诱导雄性 C57/Bl6 小鼠肥胖。用 Moro 和 Navelina 果汁代替水。还分析了 Moro 橙子中富含花色苷的提取物或纯化的矢车菊素-3-葡萄糖苷（C3G）的效果。在 12 周的时间内定期测量体重和食物摄入量。称重并分析脂肪垫的组织学；还分离总 RNA 进行微阵列分析。
无论由于含糖量增加而导致的能量摄入增加，Moro 果汁的饮食补充都能显著减少体重增加和脂肪积累，但 Navelina 果汁不能。此外，饮用 Moro 果汁的小鼠对 HFD 诱导的肥胖具有抵抗力，食物摄入量没有变化。只有花青素提取物，而不是纯化的 C3G，对脂肪积累有轻微影响。脂肪组织的高通量基因表达分析证实，Moro 果汁可以完全挽救高脂肪引起的转录重编程。
Moro 果汁对脂肪积累的抗肥胖作用不能仅归因于其花青素含量。我们的研究结果表明，Moro 橙汁中存在的多种成分可能协同作用抑制脂肪积累。
