Department of Nuclear Medicine & PET Research, VU University Medical Centre, Amsterdam, The Netherlands.
J Nucl Cardiol. 2010 Apr;17(2):264-75. doi: 10.1007/s12350-009-9181-y. Epub 2009 Dec 29.
Measuring the rate of clearance of carbon-11 labelled acetate from myocardium using positron emission tomography (PET) is an accepted technique for noninvasively assessing myocardial oxygen consumption. Initial myocardial uptake of [(11)C]acetate, however, is related to myocardial blood flow (MBF) and several tracer kinetic models for quantifying MBF using [(11)C]acetate have been proposed. The objective of this study was to assess these models.
Eighteen healthy subjects and 18 patients with hypertrophic cardiomyopathy (HCM) were studied under baseline conditions with [(11)C]acetate and [(15)O]water. Four previously reported methods, including single- and multi-tissue compartment models, were used to calculate MBF from the measured [(11)C]acetate rate of influx K (1) and the (previously) reported relationship between K (1) and MBF. These MBF values were then compared with those derived from corresponding [(15)O]water studies.
For all models, correlations between [(11)C]acetate and [(15)O]water-derived MBF ranged from .67 to .86 (all P < .005) in the control group and from .73 to .85 (all P < .001) in the HCM group. Two out of four models systematically underestimated perfusion with [(11)C]acetate, whilst the third model resulted in an overestimation. The fourth model, based on a simple single tissue compartment model with spillover, partial volume and recirculating metabolite corrections, resulted in a regression equation with a slope of near unity and an Y-intercept of almost zero (controls, K(1) = .74[MBF] + .09, r = .86, SEE = .13, P < .001 and HCM, K(1) = .89[MBF] + .03, r = .85, SEE = .12, P < .001).
[(11)C]acetate enables quantification of MBF in fairly good agreement with actual MBF in both healthy individuals and patients with HCM. A single tissue compartment model with standardized correction for recirculating metabolites and with corrections for partial volume and spillover provided the best results.
使用正电子发射断层扫描（PET）测量碳-11 标记的醋酸盐从心肌中的清除率，是一种非侵入性评估心肌耗氧量的方法。然而，初始心肌对 [(11)C]醋酸盐的摄取与心肌血流（MBF）有关，已经提出了几种使用 [(11)C]醋酸盐定量 MBF 的示踪动力学模型。本研究的目的是评估这些模型。
在 [(11)C]醋酸盐和 [(15)O]水的基础条件下，对 18 名健康受试者和 18 名肥厚型心肌病（HCM）患者进行研究。使用四种先前报道的方法，包括单组织和多组织隔室模型，从测量的 [(11)C]醋酸盐流入率 K (1) 和之前报道的 K (1) 与 MBF 之间的关系，计算 MBF。然后将这些 MBF 值与相应的 [(15)O]水研究进行比较。
对于所有模型，在对照组中，[(11)C]醋酸盐与 [(15)O]水衍生的 MBF 之间的相关性范围从.67 到.86（所有 P <.005），在 HCM 组中从.73 到.85（所有 P <.001）。四种模型中的两种系统地低估了 [(11)C]醋酸盐的灌注，而第三种模型则导致高估。基于带有溢出、部分容积和再循环代谢物校正的简单单组织隔室模型的第四种模型，产生了一条斜率接近 1、Y 截距几乎为零的回归方程（对照组，K(1) =.74[MBF] +.09，r =.86，SEE =.13，P <.001，HCM，K(1) =.89[MBF] +.03，r =.85，SEE =.12，P <.001）。
[(11)C]醋酸盐能够定量测量 MBF，与健康个体和 HCM 患者的实际 MBF 相当吻合。使用标准化的再循环代谢物校正、部分容积和溢出校正的单组织隔室模型提供了最佳结果。
