Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
BMC Genomics. 2010 Jan 11;11:19. doi: 10.1186/1471-2164-11-19.
Transmembrane receptor kinases play critical roles in both animal and plant signaling pathways regulating growth, development, differentiation, cell death, and pathogenic defense responses. In Arabidopsis thaliana, there are at least 223 Leucine-rich repeat receptor-like kinases (LRR-RLKs), representing one of the largest protein families. Although functional roles for a handful of LRR-RLKs have been revealed, the functions of the majority of members in this protein family have not been elucidated.
As a resource for the in-depth analysis of this important protein family, the complementary DNA sequences (cDNAs) of 194 LRR-RLKs were cloned into the Gateway donor vector pDONR/Zeo and analyzed by DNA sequencing. Among them, 157 clones showed sequences identical to the predictions in the Arabidopsis sequence resource, TAIR8. The other 37 cDNAs showed gene structures distinct from the predictions of TAIR8, which was mainly caused by alternative splicing of pre-mRNA. Most of the genes have been further cloned into Gateway destination vectors with GFP or FLAG epitope tags and have been transformed into Arabidopsis for in planta functional analysis. All clones from this study have been submitted to the Arabidopsis Biological Resource Center (ABRC) at Ohio State University for full accessibility by the Arabidopsis research community.
Most of the Arabidopsis LRR-RLK genes have been isolated and the sequence analysis showed a number of alternatively spliced variants. The generated resources, including cDNA entry clones, expression constructs and transgenic plants, will facilitate further functional analysis of the members of this important gene family.
跨膜受体激酶在动植物信号通路中发挥着关键作用，调节生长、发育、分化、细胞死亡和病原防御反应。在拟南芥中，至少有 223 个富含亮氨酸重复受体样激酶（LRR-RLK），代表最大的蛋白质家族之一。尽管已经揭示了少数 LRR-RLK 的功能作用，但该蛋白质家族的大多数成员的功能尚未阐明。
作为深入分析这个重要蛋白质家族的资源，194 个 LRR-RLK 的 cDNA 被克隆到 Gateway 供体载体 pDONR/Zeo 中，并通过 DNA 测序进行分析。其中，157 个克隆显示出与拟南芥序列资源 TAIR8 预测序列完全相同的序列。其他 37 个 cDNA 显示出与 TAIR8 预测不同的基因结构，这主要是由于前体 mRNA 的选择性剪接造成的。大多数基因已进一步克隆到带有 GFP 或 FLAG 表位标签的 Gateway 目的载体中，并转化为拟南芥进行体内功能分析。本研究的所有克隆已提交给俄亥俄州立大学的拟南芥生物资源中心（ABRC），供整个拟南芥研究界充分访问。
大多数拟南芥 LRR-RLK 基因已经被分离出来，序列分析显示出许多选择性剪接的变体。生成的资源，包括 cDNA 入口克隆、表达构建体和转基因植物，将促进对这个重要基因家族成员的进一步功能分析。
