Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Ireland.
Gene. 2010 Mar 15;453(1-2):9-23. doi: 10.1016/j.gene.2009.12.007. Epub 2010 Jan 11.
Ardmore is a novel mycobacteriophage isolated from a soil sample collected in County Waterford, Ireland. The genome of this phage has been fully sequenced and is composed of 52,141 bp of linear double stranded DNA with a GC content of 61.49%. 88 ORFs were identified of which 34 were assigned predicted functions based on their homology to previously characterised proteins, their location in the genome, computer-predicted structural characteristics or presence of conserved motifs in their sequence. The Ardmore genome appears highly similar to mycobacteriophages Fruitloop and Tweety with BLASTn analysis showing 87% and 80% identity respectively. A predicted beta-lactamase gene was detected in the sequence, and an unusual +1 frameshift event for the translation of tail genes was also observed.
阿德莫尔是一种从爱尔兰沃特福德郡采集的土壤样本中分离出来的新型分枝杆菌噬菌体。该噬菌体的基因组已被完全测序，由 52141 个碱基对的线性双链 DNA 组成，GC 含量为 61.49%。鉴定出 88 个 ORF，其中 34 个根据其与先前表征的蛋白质的同源性、在基因组中的位置、计算机预测的结构特征或序列中保守基序的存在，被赋予了预测功能。阿德莫尔基因组与分枝杆菌噬菌体 Fruitloop 和 Tweety 非常相似，BLASTn 分析显示分别具有 87%和 80%的同一性。在序列中检测到一个预测的β-内酰胺酶基因，并且还观察到一个不寻常的翻译尾巴基因的+1 移码事件。
