阿根廷火地岛乌斯怀亚地区利用脱落的急性期抗原对先天性克氏锥虫感染的早期诊断。
Early diagnosis of congenital Trypanosoma cruzi infection, using shed acute phase antigen, in Ushuaia, Tierra del Fuego, Argentina.
机构信息
Laboratorio Central, Hospital Regional Ushuaia, Centro Nacional de Diagnóstico e Investigación de Endemoepidemias (CeNDIE) ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud, Instituto de Efectividad Clínica y Sanitaria, Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Buenos Aires, Argentina.
出版信息
Am J Trop Med Hyg. 2010 Jan;82(1):55-9. doi: 10.4269/ajtmh.2010.09-0219.
Abstract
Chagas' disease, or American trypanosomiasis, is caused by the protozoan parasite Trypanasoma cruzi. It is estimated that 15,000 new cases of congenital T. cruzi transmission occur in the Americas each year. The aim of this study was to estimate the rate of congenital T. cruzi infection in infants born to infected women living in Ushuaia, Argentina, as well to assess a serologic test using Shed Acute Phase Antigen (SAPA) for a timely diagnosis of congenital infection. The rate of congenital infection among children in the study was 4.4% (3/68). Our results show that for infants younger than 30 days of age, matched blood samples from mother and infant were capable of identifying congenital transmission of infection using an enzyme-linked immunosorbent assay with SAPA. For infants older than 3 months, congenital infection could be ruled out using the same procedure.
摘要
恰加斯病，又称美洲锥虫病，是由原生动物寄生虫克氏锥虫引起的。据估计，每年美洲有 15000 例先天性克氏锥虫传播的新病例。本研究旨在评估阿根廷乌斯怀亚感染妇女所生婴儿先天性 T. cruzi 感染的发生率，并评估使用 Shed Acute Phase Antigen（SAPA）的血清学检测方法进行先天性感染的及时诊断。研究中儿童的先天性感染率为 4.4%（3/68）。我们的结果表明，对于 30 天龄以下的婴儿，使用 SAPA 酶联免疫吸附试验可以从母亲和婴儿匹配的血样中鉴定出先天性感染的传播。对于 3 个月以上的婴儿，可以使用相同的程序排除先天性感染。
相似文献
1
Early diagnosis of congenital Trypanosoma cruzi infection, using shed acute phase antigen, in Ushuaia, Tierra del Fuego, Argentina.
Am J Trop Med Hyg. 2010 Jan;82(1):55-9. doi: 10.4269/ajtmh.2010.09-0219.
2
Diagnosis of congenital Trypanosoma cruzi infection: A serologic test using Shed Acute Phase Antigen (SAPA) in mother-child binomial samples.
Acta Trop. 2015 Jul;147:31-7. doi: 10.1016/j.actatropica.2015.03.026. Epub 2015 Apr 3.
3
Congenital transmission of Trypanosoma cruzi in non-endemic areas: evaluation of a screening program in a tertiary care hospital in Barcelona, Spain.
Am J Trop Med Hyg. 2012 Nov;87(5):832-6. doi: 10.4269/ajtmh.2012.12-0152. Epub 2012 Sep 17.
4
High prevalence of congenital Trypanosoma cruzi infection and family clustering in Salta, Argentina.
Pediatrics. 2005 Jun;115(6):e668-72. doi: 10.1542/peds.2004-1732.
5
Trypomastigote Excretory Secretory Antigen Blot Is Associated With Trypanosoma cruzi Load and Detects Congenital T. cruzi Infection in Neonates, Using Anti-Shed Acute Phase Antigen Immunoglobulin M.
J Infect Dis. 2019 Jan 29;219(4):609-618. doi: 10.1093/infdis/jiy562.
6
Predictive role of polymerase chain reaction in the early diagnosis of congenital Trypanosoma cruzi infection.
Acta Trop. 2014 Sep;137:195-200. doi: 10.1016/j.actatropica.2014.05.016. Epub 2014 Jun 2.
7
The Immunoglobulin M-Shed Acute Phase Antigen (SAPA)-test for the Early Diagnosis of Congenital Chagas Disease in the Time of the Elimination Goal of Mother-to-Child Transmission.
Clin Infect Dis. 2021 Jul 15;73(2):e477-e484. doi: 10.1093/cid/ciaa986.
8
[Maternal factors associated to congenital transmission of Chagas disease in children born and siblings in Chaco province, Argentina.].
Rev Fac Cien Med Univ Nac Cordoba. 2018 Dec 27;75(4):279-287. doi: 10.31053/1853.0605.v75.n4.21260.
9
[Immunoenzymatic evaluation of the recombinant SAPA protein of Trypanosoma cruzi in naturally infected dogs].
Rev Argent Microbiol. 2012 Jul-Sep;44(3):177-81.
10
Serological diagnosis of Trypanosoma rangeli infected patients. A comparison of different methods and its implications for the diagnosis of Chagas' disease.
Scand J Immunol. 1997 Mar;45(3):322-30. doi: 10.1046/j.1365-3083.1997.d01-405.x.
引用本文的文献
1
Mass spectrometry-based discovery and diagnostic validation of T. cruzi antigens in the urine of congenitally infected Chagas Disease patients.
PLoS Negl Trop Dis. 2025 Jun 16;19(6):e0013082. doi: 10.1371/journal.pntd.0013082. eCollection 2025 Jun.
2
Congenital Chagas disease: A cohort study to assess molecular diagnostic methods at the Chagas disease national reference center of Argentina.
PLoS Negl Trop Dis. 2025 Jan 10;19(1):e0012785. doi: 10.1371/journal.pntd.0012785. eCollection 2025 Jan.
3
Use of sera cell free DNA (cfDNA) and exovesicle-DNA for the molecular diagnosis of chronic Chagas disease.
PLoS One. 2023 Sep 8;18(9):e0282814. doi: 10.1371/journal.pone.0282814. eCollection 2023.
4
Estimation of the morbidity and mortality of congenital Chagas disease: A systematic review and meta-analysis.
PLoS Negl Trop Dis. 2022 Nov 7;16(11):e0010376. doi: 10.1371/journal.pntd.0010376. eCollection 2022 Nov.
5
Comparing in vivo bioluminescence imaging and the Multi-Cruzi immunoassay platform to develop improved Chagas disease diagnostic procedures and biomarkers for monitoring parasitological cure.
PLoS Negl Trop Dis. 2022 Oct 3;16(10):e0010827. doi: 10.1371/journal.pntd.0010827. eCollection 2022 Oct.
6
Infection at the Maternal-Fetal Interface: Implications of Parasite Load in the Congenital Transmission and Challenges in the Diagnosis of Infected Newborns.
Front Microbiol. 2019 Jun 7;10:1250. doi: 10.3389/fmicb.2019.01250. eCollection 2019.
7
Trypanosoma cruzi infection in transfusion medicine.
Hematol Transfus Cell Ther. 2019 Jul-Sep;41(3):262-267. doi: 10.1016/j.htct.2018.12.001. Epub 2019 Mar 21.
8
The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America.
BMJ Glob Health. 2018 Oct 11;3(5):e001069. doi: 10.1136/bmjgh-2018-001069. eCollection 2018.
9
Trypomastigote Excretory Secretory Antigen Blot Is Associated With Trypanosoma cruzi Load and Detects Congenital T. cruzi Infection in Neonates, Using Anti-Shed Acute Phase Antigen Immunoglobulin M.
J Infect Dis. 2019 Jan 29;219(4):609-618. doi: 10.1093/infdis/jiy562.
10
Chagas Disease Diagnostic Applications: Present Knowledge and Future Steps.
Adv Parasitol. 2017;97:1-45. doi: 10.1016/bs.apar.2016.10.001. Epub 2016 Nov 14.
本文引用的文献
1
The value of molecular studies for the diagnosis of congenital Chagas disease in northeastern Argentina.
Am J Trop Med Hyg. 2008 Apr;78(4):624-7.
2
Mother-to-child transmission of Chagas' disease in North America: why don't we do more?
Matern Child Health J. 2008 May;12(3):283-6. doi: 10.1007/s10995-007-0246-8. Epub 2007 Jun 30.
3
Evidence of congenital transmission of Trypanosoma cruzi in a vector-free area of Bolivia.
Trans R Soc Trop Med Hyg. 2007 Nov;101(11):1159-60. doi: 10.1016/j.trstmh.2007.03.015. Epub 2007 May 17.
4
Early diagnosis of congenital Trypanosoma cruzi infection using PCR, hemoculture, and capillary concentration, as compared with delayed serology.
J Parasitol. 2005 Dec;91(6):1468-73. doi: 10.1645/GE-549R.1.
5
[Implementation and evaluation of a locally sustainable system of prenatal diagnosis to detect cases of congenital Chagas disease in endemic areas of Paraguay].
Rev Soc Bras Med Trop. 2005;38 Suppl 2:49-54.
6
[Congenital transmission of Trypanosoma cruzi infection in Argentina].
Rev Soc Bras Med Trop. 2005;38 Suppl 2:29-32.
7
Diagnosis of Chagas disease using recombinant DNA technology.
Parasitol Today. 1990 Apr;6(4):137-9. doi: 10.1016/0169-4758(90)90234-u.
8
Congenital infection with Trypanosoma cruzi: from mechanisms of transmission to strategies for diagnosis and control.
Rev Soc Bras Med Trop. 2003 Nov-Dec;36(6):767-71. doi: 10.1590/s0037-86822003000600024.
9
Aetiological treatment of congenital Chagas' disease diagnosed and monitored by the polymerase chain reaction.
J Antimicrob Chemother. 2003 Sep;52(3):441-9. doi: 10.1093/jac/dkg338. Epub 2003 Aug 13.
10
Congenital transmission of Trypanosoma cruzi infection in Argentina.
Emerg Infect Dis. 2003 Jan;9(1):29-32. doi: 10.3201/eid0901.020274.
Zotero 插件