阿根廷火地岛乌斯怀亚地区利用脱落的急性期抗原对先天性克氏锥虫感染的早期诊断。
Early diagnosis of congenital Trypanosoma cruzi infection, using shed acute phase antigen, in Ushuaia, Tierra del Fuego, Argentina.
机构信息
Laboratorio Central, Hospital Regional Ushuaia, Centro Nacional de Diagnóstico e Investigación de Endemoepidemias (CeNDIE) ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud, Instituto de Efectividad Clínica y Sanitaria, Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Buenos Aires, Argentina.
出版信息
Am J Trop Med Hyg. 2010 Jan;82(1):55-9. doi: 10.4269/ajtmh.2010.09-0219.
Abstract
Chagas' disease, or American trypanosomiasis, is caused by the protozoan parasite Trypanasoma cruzi. It is estimated that 15,000 new cases of congenital T. cruzi transmission occur in the Americas each year. The aim of this study was to estimate the rate of congenital T. cruzi infection in infants born to infected women living in Ushuaia, Argentina, as well to assess a serologic test using Shed Acute Phase Antigen (SAPA) for a timely diagnosis of congenital infection. The rate of congenital infection among children in the study was 4.4% (3/68). Our results show that for infants younger than 30 days of age, matched blood samples from mother and infant were capable of identifying congenital transmission of infection using an enzyme-linked immunosorbent assay with SAPA. For infants older than 3 months, congenital infection could be ruled out using the same procedure.
摘要
恰加斯病，又称美洲锥虫病，是由原生动物寄生虫克氏锥虫引起的。据估计，每年美洲有 15000 例先天性克氏锥虫传播的新病例。本研究旨在评估阿根廷乌斯怀亚感染妇女所生婴儿先天性 T. cruzi 感染的发生率，并评估使用 Shed Acute Phase Antigen（SAPA）的血清学检测方法进行先天性感染的及时诊断。研究中儿童的先天性感染率为 4.4%（3/68）。我们的结果表明，对于 30 天龄以下的婴儿，使用 SAPA 酶联免疫吸附试验可以从母亲和婴儿匹配的血样中鉴定出先天性感染的传播。对于 3 个月以上的婴儿，可以使用相同的程序排除先天性感染。
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