Department of Animal Sciences, University of Illinois, Urbana, 61801, USA.
Funct Integr Genomics. 2010 Mar;10(1):53-61. doi: 10.1007/s10142-009-0154-7.
Our objectives were to compare gene expression profiles in blood polymorphonuclear cells (PMN) during a Streptococcus uberis intramammary challenge between lactating cows subjected to feed restriction to induce negative energy balance (NEB; n=5) and cows fed ad libitum to maintain positive energy balance (PEB; n=5). After 5 days of feed restriction, one rear mammary quarter of each cow was inoculated with 5,000 cfu of S. uberis. Blood PMN were isolated at 24 h post-inoculation from all cows for mRNA expression via quantitative polymerase chain reaction for 20 genes associated with immune response and metabolism. A total of 12 genes were differentially expressed in blood PMN in NEB versus PEB cows. Upregulated genes by NEB were ALOX5AP, CPNE3, IL1R2, IL6, TLR2, TLR4, and THY1, and downregulated genes were HLA-DRA, HLA-A, IRAK1, SOD1, and TNF. Network analysis revealed that TNF was associated with several of the affected genes in NEB cows compared with PEB cows. Results showed that 24 h after intramammary challenge with S. uberis, cows in NEB had altered PMN expression of genes involved with immune response. Our data provide new information on transcriptomic mechanisms associated with NEB and the corresponding inhibition of immune response in lactating dairy cows.
我们的目的是比较泌乳奶牛在受到饲料限制以诱导负能平衡（NEB；n=5）和自由采食以维持正能平衡（PEB；n=5）时，在链球菌乳房内感染期间血液多形核细胞（PMN）中的基因表达谱。在饲料限制 5 天后，每头牛的后一个乳房象限都接种了 5000 cfu 的 S. uberis。在接种后 24 小时，从所有奶牛中分离血液 PMN，通过定量聚合酶链反应对与免疫反应和代谢相关的 20 个基因进行 mRNA 表达。NEB 与 PEB 奶牛相比，血液 PMN 中有 12 个基因表达差异。NEB 上调的基因有 ALOX5AP、CPNE3、IL1R2、IL6、TLR2、TLR4 和 THY1，下调的基因有 HLA-DRA、HLA-A、IRAK1、SOD1 和 TNF。网络分析显示，与 PEB 奶牛相比，TNF 与 NEB 奶牛中受影响的几个基因有关。结果表明，在 S. uberis 乳房内感染后 24 小时，NEB 奶牛的 PMN 中与免疫反应相关的基因表达发生改变。我们的数据提供了与 NEB 相关的转录组机制以及泌乳奶牛免疫反应相应抑制的新信息。
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