Montuschi Paolo, Barnes Peter J, Ciabattoni Giovanni
Department of Pharmacology, Catholic University of the Sacred Heart, Rome, Italy.
Methods Mol Biol. 2010;594:73-84. doi: 10.1007/978-1-60761-411-1_5.
Oxidative stress is functionally involved in the pathophysiology of lung diseases including asthma and chronic obstructive pulmonary disease. 8-Isoprostane, which is derived from free radical-catalyzed peroxidation of arachidonic acid, is one of the most reliable biomarkers of oxidative stress. Exhaled breath condensate (EBC) is a completely noninvasive method for collecting airway secretions. We developed a specific and sensitive radioimmunoassay (RIA) that has been applied to the measurement of 8-isoprostane in EBC. This RIA for 8-isoprostane has been validated using high performance liquid chromatography. Measurement of 8-isoprostane in EBC is a useful noninvasive technique for exploring the role of oxidative stress in lung diseases. This technique might provide important insights into the understanding of the clinical pharmacology of antioxidants and might be useful for monitoring the effects of pharmacological therapy.
氧化应激在包括哮喘和慢性阻塞性肺疾病在内的肺部疾病的病理生理学中发挥着功能性作用。8-异前列腺素是由自由基催化的花生四烯酸过氧化反应产生的，是氧化应激最可靠的生物标志物之一。呼出气冷凝物（EBC）是一种收集气道分泌物的完全非侵入性方法。我们开发了一种特异性和敏感性均高的放射免疫分析方法（RIA），该方法已应用于EBC中8-异前列腺素的测定。这种用于8-异前列腺素的RIA已通过高效液相色谱法进行了验证。测定EBC中的8-异前列腺素是一种用于探索氧化应激在肺部疾病中作用的有用的非侵入性技术。该技术可能为理解抗氧化剂的临床药理学提供重要见解，并且可能有助于监测药物治疗的效果。
