Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK.
J Mater Sci Mater Med. 2010 Aug;21(8):2293-8. doi: 10.1007/s10856-009-3964-1. Epub 2009 Dec 20.
Bone regeneration requires scaffolds that possess suitable mechanical and biological properties. This study sought to develop a novel collagen-nHA biocomposite scaffold via two new methods. Firstly a stable nHA suspension was produced and added to a collagen slurry (suspension method), and secondly, porous collagen scaffolds were immersed in nHA suspension after freeze-drying (immersion method). Significantly stronger constructs were produced using both methods compared to collagen only scaffolds, with a high porosity maintained (>98.9%). It was found that Coll-nHA composite scaffolds produced by the suspension method were up to 18 times stiffer than the collagen control (5.50 +/- 1.70 kPa vs. 0.30 +/- 0.09 kPa). The suspension method was also more reproducible, and the quantity of nHA incorporated could be varied with greater ease than with the immersion technique. In addition, Coll-nHA composites display excellent biological activity, demonstrating their potential as bone graft substitutes in orthopaedic regenerative medicine.
骨再生需要具有合适机械和生物特性的支架。本研究旨在通过两种新方法开发一种新型胶原-nHA 生物复合材料支架。首先，制备了稳定的 nHA 悬浮液并添加到胶原浆液中（悬浮法），其次，将多孔胶原支架在冷冻干燥后浸入 nHA 悬浮液中（浸渍法）。与仅用胶原制备的支架相比，这两种方法都能产生明显更强的构建体，且保持高孔隙率（>98.9%）。结果发现，与胶原对照组相比，通过悬浮法制备的 Coll-nHA 复合材料支架的刚性高 18 倍（5.50 +/- 1.70 kPa 比 0.30 +/- 0.09 kPa）。悬浮法也更具重现性，并且与浸渍技术相比，更易于改变 nHA 的添加量。此外，Coll-nHA 复合材料具有优异的生物活性，表明它们有可能成为骨科再生医学中的骨移植替代物。
