肾细胞癌中烟酰胺 N-甲基转移酶蛋白的表达。
Nicotinamide N-methyltransferase protein expression in renal cell cancer.
机构信息
Department of Clinical Laboratory, Sir Run Run Show Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
出版信息
J Zhejiang Univ Sci B. 2010 Feb;11(2):136-43. doi: 10.1631/jzus.B0900249.
Abstract
OBJECTIVE
To understand the function of nicotinamide N-methyltransferase (NNMT) protein as tumor biomarker in renal carcinoma.
METHODS
Recombinant NNMT protein was used to prepare monoclonal antibodies by hybridoma technique. The diagnostic and prognostic function of NNMT protein in renal carcinoma was evaluated by analyzing 74 renal cancer tissues through immunohistochemical staining for NNMT by using the prepared antibodies.
RESULTS
Two hybridomas named 2F8 and 1E7 stably secreting the monoclonal antibodies were isolated successfully, and characters such as isotypes and specificity were determined. NNMT protein was significantly up-regulated in renal cancer and significantly associated with tumor histology and ages. The univariate survival analysis demonstrated that the pT-status, high levels of NNMT, and distant metastasis were significant prognosticators.
CONCLUSION
NNMT is over-expressed in a large proportion in renal cell cancers. High NNMT expression is significantly associated with unfavorable prognosis. However, the prognostic value of NNMT needs further verification in larger sample sizes.
摘要
目的
了解烟酰胺 N-甲基转移酶（NNMT）蛋白作为肾癌肿瘤标志物的功能。
方法
通过杂交瘤技术使用重组 NNMT 蛋白制备单克隆抗体。通过用制备的抗体对 74 例肾癌组织进行免疫组织化学染色，分析 NNMT 蛋白在肾癌中的诊断和预后功能。
结果
成功分离出稳定分泌单克隆抗体的两个杂交瘤细胞株 2F8 和 1E7，并确定了其同型和特异性等特征。NNMT 蛋白在肾癌中显著上调，与肿瘤组织学和年龄显著相关。单因素生存分析表明，pT 分期、NNMT 水平高和远处转移是显著的预后因素。
结论
NNMT 在很大比例的肾细胞癌中过度表达。NNMT 高表达与不良预后显著相关。然而，NNMT 的预后价值需要在更大的样本量中进一步验证。
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