Department of Rheumatology, Zhongnan Hospital, Medical College of Wuhan University, Wuhan, China.
J Clin Immunol. 2010 Mar;30(2):221-5. doi: 10.1007/s10875-009-9365-x. Epub 2010 Jan 28.
To investigate the role of interleukin-17A (IL-17A) and Th17 cell in the pathogenesis of systemic lupus erythematosus (SLE), we studied the plasma IL-17A and the expression of Th17 cell transcription factor RORgammat in Chinese new-onset SLE patients.
Sixty SLE patients aged between 18 and 40 years and 56 age-matched healthy volunteers were involved in the study. Enzyme-linked immunosorbent assay was used to measure plasma IL-17A level, and rea1-time fluorescent quantitative polymerase chain reaction was used to measure RORgammat mRNA.
The results showed that both IL-17A level and RORgammat mRNA in SLE patients were higher than that of controls. Correlation analysis indicated that plasma IL-17A level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index, not with RORgammat mRNA.
We concluded that IL-17A might play a role in the pathogenesis of SLE and associated with disease activity. RORgammat-determined Th17 cell might be involved with increased IL-17A in SLE but not exclusively the unique source.
为了研究白细胞介素-17A（IL-17A）和 Th17 细胞在系统性红斑狼疮（SLE）发病机制中的作用，我们研究了中国新发 SLE 患者的血浆 IL-17A 和 Th17 细胞转录因子 RORgammat 的表达。
本研究纳入了 60 名 18-40 岁的 SLE 患者和 56 名年龄匹配的健康志愿者。采用酶联免疫吸附试验检测血浆 IL-17A 水平，实时荧光定量聚合酶链反应检测 RORgammat mRNA。
结果表明，SLE 患者的 IL-17A 水平和 RORgammat mRNA 均高于对照组。相关性分析表明，血浆 IL-17A 水平与系统性红斑狼疮疾病活动指数呈正相关，与 RORgammat mRNA 无关。
我们得出结论，IL-17A 可能在 SLE 的发病机制中发挥作用，并与疾病活动相关。RORgammat 决定的 Th17 细胞可能与 SLE 中 IL-17A 的增加有关，但不是唯一的来源。
