College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Molecules. 2010 Jan 19;15(1):399-406. doi: 10.3390/molecules15010399.
Protopanaxatriol saponins obtained with AB-8 macroporous resin mainly consisted of ginsenosides Rg(1) and Re. A novel mono-ester of ginsenoside-Rh(1) (ginsenoside-ORh(1)) was synthesized through further enzymatic hydrolysis and octanoyl chloride modifications. A 53% yield was obtained by a facile synthetic method. The structures were identified on the basis of 1D-NMR and 2D-NMR, as well as ESI-TOF-MS mass spectroscopic analyses. The isolated and synthetic compounds were applied in an anti-tumor bioassay, in which ginsenoside ORh(1) showed moderate effects on Murine H22 Hepatoma Cells.
AB-8 大孔树脂提取的原人参三醇皂苷主要由人参皂苷 Rg(1)和 Re 组成。通过进一步的酶解和辛酰氯修饰，合成了一种新型的人参皂苷-Rh(1)的单酯（人参皂苷-ORh(1)）。通过一种简便的合成方法，获得了 53%的产率。结构通过 1D-NMR 和 2D-NMR 以及 ESI-TOF-MS 质谱分析确定。分离得到的化合物和合成化合物都应用于抗肿瘤生物测定中，其中人参皂苷 ORh(1)对小鼠 H22 肝癌细胞表现出中等的作用。
