S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):654-664. doi: 10.1099/ijs.0.031898-0. Epub 2011 May 6.
An aerobic, pink-pigmented, chemo-organotrophic bacterium, designated strain SN10(T), was isolated from a methanotrophic enrichment culture obtained from an acidic Sphagnum peat. This isolate was represented by Gram-negative, non-motile rods that multiply by normal cell division and form rosettes. Strain SN10(T) is an obligately acidophilic, mesophilic bacterium capable of growth at pH 3.2-6.6 (with an optimum at pH 4.7-5.2) and at 6-32 °C (with an optimum at 20-24 °C). The preferred growth substrates are sugars and several heteropolysaccharides of plant and microbial origin, such as pectin, lichenan, fucoidan and gellan gum. While not being capable of growth on C(1) compounds, strain SN10(T) can develop in co-culture with exopolysaccharide-producing methanotrophs by utilization of their capsular material. The major fatty acids determined in strain SN10(T) using the conventional lipid extraction procedure are iso-C(15:0) and C(16:1)ω7c. Upon hydrolysis of total cell material, substantial amounts of the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (isodiabolic acid) were also detected. The polar lipids are two phosphohexoses, phosphatidylethanolamine, phosphatidylglycerol and several phospholipids of unknown structure. The major quinone is MK-8. Pigments are carotenoids. The G+C content of the DNA is 60.7 mol%. Strain SN10(T) forms a separate lineage within subdivision 1 of the phylum Acidobacteria and displays 94.0-95.4% 16S rRNA gene sequence similarity to members of the genera Edaphobacter and Granulicella, 93.0-93.7% similarity to members of the genus Terriglobus and 92.2-92.3 % similarity to the type strains of Telmatobacter bradus and Acidobacterium capsulatum. Therefore, strain SN10(T) is classified within a novel genus and species, for which the name Bryocella elongata gen. nov., sp. nov. is proposed. Strain SN10(T) (=LMG 25276(T) =DSM 22489(T)) is the type strain of Bryocella elongata. An emended description of Edaphobacter aggregans Koch et al. 2008 is also given.
一株好氧、粉红色、化能有机营养型细菌，被命名为 SN10(T) 菌株，从酸性泥炭沼泽的甲烷营养富集培养物中分离得到。该分离株由革兰氏阴性、非运动的杆菌组成，通过正常细胞分裂繁殖并形成玫瑰花结。SN10(T) 菌株是一种专性嗜酸、嗜中温细菌，能够在 pH3.2-6.6（最佳 pH4.7-5.2）和 6-32°C（最佳 20-24°C）下生长。其最适生长底物为糖和几种植物和微生物来源的杂多糖，如果胶、lichenan、褐藻酸和凝胶多糖。尽管不能在 C1 化合物上生长，但 SN10(T) 菌株可以通过利用其荚膜物质与产胞外多糖的甲烷营养菌共培养而生长。使用常规脂质提取程序在 SN10(T) 菌株中确定的主要脂肪酸为 iso-C15:0 和 C16:1ω7c。在总细胞物质水解后，还检测到大量罕见的膜贯穿脂质 13,16-二甲基辛二酸（异二酮酸）。极性脂类为两种磷酸己糖、磷脂酰乙醇胺、磷脂酰甘油和几种结构未知的磷脂。主要醌为 MK-8。色素为类胡萝卜素。DNA 的 G+C 含量为 60.7 mol%。SN10(T) 菌株在酸杆菌门的 1 亚群中形成一个单独的谱系，与 Edaphobacter 和 Granulicella 属的成员具有 94.0-95.4%的 16S rRNA 基因序列相似性，与 Terriglobus 属的成员具有 93.0-93.7%的相似性，与 Telmatobacter bradus 和 Acidobacterium capsulatum 的模式菌株具有 92.2-92.3%的相似性。因此，SN10(T) 菌株被分类为一个新属和新种，建议将其命名为 Bryocella elongata gen. nov.，sp. nov.。SN10(T) 菌株（=LMG 25276(T)=DSM 22489(T)）是 Bryocella elongata 的模式菌株。还给出了 Edaphobacter aggregans Koch 等人的修订描述。2008 年。
