Park Daae, Kim Hyoung Ja, Jung Seo Yun, Yook Chang-Soo, Jin Changbae, Lee Yong Sup
Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, 1 Hoegi-Dong, Seoul 130-701, Korea.
Chem Pharm Bull (Tokyo). 2010 Feb;58(2):238-41. doi: 10.1248/cpb.58.238.
To search for secondary metabolites of Alnus hirsuta (Betulaceae), various chromatographic separations of the ethyl acetate soluble fraction of the stem bark of A. hirsuta led to the isolation of a new diarylheptanoid glycoside, (3R)-1,7-bis-(4-dihydroxyphenyl)-3-heptanol 3-O-beta-D-glucopyranosyl(1-->3)-beta-D-xylopyranoside (13) and twelve diarylheptanoid derivatives, namely, oregonin (1), rubranoside A (2), hirsutanonol 5-O-beta-D-glucopyranoside (3), rubranoside B (4), rubranoside C (5), hirsutanonol (6), hirsutenone (7), (5S)-O-methylhirsutanonol (8), platyphylloside (9), platyphyllonol 5-O-beta-D-xylopyranoside (10), aceroside VII (11) and platyphyllenone (12). Isolates were assessed for their hepatoprotective effects against tert-butylhydroperoxide (t-BHP)-induced toxicity in HepG2 cells. Of these isolates, compounds 1-8 showed significant hepatoprotective effects on t-BHP-induced damage to HepG2 cells, with 8 exhibiting the greatest protective effect (50.7 + or - 3.7% at a concentration of 10 microM).
为了寻找毛赤杨（桦木科）的次生代谢产物，对毛赤杨茎皮乙酸乙酯可溶部分进行了各种色谱分离，从中分离出一种新的二芳基庚烷糖苷，(3R)-1,7-双-(4-二羟基苯基)-3-庚醇3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃木糖苷（13）以及十二种二芳基庚烷衍生物，即俄勒冈宁（1）、红杉糖苷A（2）、毛赤杨醇5-O-β-D-吡喃葡萄糖苷（3）、红杉糖苷B（4）、红杉糖苷C（5）、毛赤杨醇（6）、毛赤杨烯酮（7）、(5S)-O-甲基毛赤杨醇（8）、宽叶山萮菜苷（9）、宽叶山萮菜醇5-O-β-D-吡喃木糖苷（10）、槭树苷VII（11）和宽叶山萮菜烯酮（12）。评估了这些分离物对叔丁基过氧化氢（t-BHP）诱导的HepG2细胞毒性的肝保护作用。在这些分离物中，化合物1-8对t-BHP诱导的HepG2细胞损伤显示出显著的肝保护作用,其中8在浓度为μM时表现出最大的保护作用（50.7±3.7%）。
