Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital Medical University School of Stomatology, Beijing, People's Republic of China.
Stem Cells Dev. 2010 Sep;19(9):1375-83. doi: 10.1089/scd.2009.0258.
Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a novel population of postnatal stem cells capable of differentiating into neural cells, odontogenic cells, and adipocytes. SHED were reported to differentiate into neural cells based on cellular morphology and the expression of early neuronal markers when cultured under neural inductive conditions. This study therefore investigated the therapeutic efficacy of SHED in alleviating Parkinson's disease (PD) in a rat model. We found that SHED could be induced to form neural-like spheres in a medium optimized for neural stem cells in vitro. After incubation with a cocktail of cytokines including sonic hedgehog, fibroblast growth factor 8, glial cell line-derived neurotrophic factor, and forskolin, these SHED-derived spheres further differentiated into a cell population that contained specific dopaminergic neurons. Moreover, transplantation of SHED spheres into the striatum of parkinsonian rats partially improved the apomorphine-evoked rotation of behavorial disorders compared to transplantation of control SHED. Our data indicate that SHED, potentially derived from neural crest cells, may be an optimal source of postnatal stem cells for PD treatment.
人脱落乳牙中的干细胞（SHED）已被鉴定为一种新型的成体干细胞，能够分化为神经细胞、成牙本质细胞和成脂细胞。有报道称，SHED 在神经诱导条件下培养时，根据细胞形态和早期神经元标志物的表达而分化为神经细胞。因此，本研究探讨了 SHED 在减轻帕金森病（PD）大鼠模型中的治疗效果。我们发现，SHED 可以在体外优化的神经干细胞培养基中诱导形成神经样球体。在用包括 sonic hedgehog、成纤维细胞生长因子 8、胶质细胞源性神经营养因子和 forskolin 的细胞因子鸡尾酒孵育后，这些 SHED 衍生的球体进一步分化为含有特定多巴胺能神经元的细胞群。此外，与移植对照 SHED 相比，将 SHED 球体移植到帕金森病大鼠的纹状体部分改善了阿扑吗啡诱发的行为障碍的旋转。我们的数据表明，SHED 可能来源于神经嵴细胞，是 PD 治疗的一种理想的成体干细胞来源。
