Molnlycke Health Care, Goteborg, Sweden.
APMIS. 2010 Feb;118(2):156-64. doi: 10.1111/j.1600-0463.2009.02580.x.
There is growing evidence that bacteria play a crucial role in the persistence of chronic wounds. These bacteria are most probably present in polymer-embedded aggregates that represent the biofilm mode of growth. Much work has been carried out to study the development of biofilms in vitro, in particular in attachment to solid surfaces. The observations from the chronic wounds indicate that the bacteria are not attached to a solid surface. Consequently, a new in vitro model is required to investigate biofilms in more wound-like settings. This study describes such a novel in vitro model, with bacteria growing as biofilm aggregates in a collagen gel matrix with serum protein mimicking the wound bed of chronic wounds. The model was verified to comprise important hallmarks of biofilms such as the bacterial embedment in a matrix and increased antibiotic tolerance. Furthermore, we have verified the relevance of the model by comparing the organization of the bacteria in the model with the organization of the bacteria in a real chronic wound. We believe that we have developed an important new model for investigating bacterial biofilms in chronic wounds. This model may be used to study biofilm development in chronic wounds and to develop novel diagnostic tools as well as treatment strategies.
越来越多的证据表明，细菌在慢性伤口的持续存在中起着至关重要的作用。这些细菌很可能存在于聚合物嵌入的聚集体中，代表生物膜的生长方式。已经进行了大量的工作来研究生物膜在体外的发展，特别是在固体表面的附着。从慢性伤口的观察结果表明，细菌没有附着在固体表面上。因此，需要一种新的体外模型来在更类似于伤口的环境中研究生物膜。本研究描述了这样一种新的体外模型，细菌在含有血清蛋白的胶原凝胶基质中生长为生物膜聚集体，模拟慢性伤口的创床。该模型被证明包含生物膜的重要特征，例如细菌在基质中的嵌入和抗生素耐药性的增加。此外，我们通过比较模型中细菌的组织与真实慢性伤口中细菌的组织，验证了该模型的相关性。我们相信我们已经开发出一种用于研究慢性伤口中细菌生物膜的重要新模型。该模型可用于研究慢性伤口中生物膜的发展，并开发新的诊断工具和治疗策略。
