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一种从海藻酸钙凝胶中获得葡萄球菌属单种和双种培养物的新方法，以及以儿茶酚胺为例研究活性化合物对这些培养物的作用。

A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines.

机构信息

Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2024 Aug;177(4):512-516. doi: 10.1007/s10517-024-06217-6. Epub 2024 Sep 12.

DOI:10.1007/s10517-024-06217-6
PMID:39266918
Abstract

A simple and efficient method for obtaining monospecies and binary Staphylococcus aureus and Staphylococcus epidermidis cultures in sodium alginate gel matrix mimicking the natural microenvironment of the nasal cavity was proposed. The cultures were used for studying the effect of norepinephrine on monospecies and binary communities of two types of bacteria, S. aureus (invasive strain) and S. epidermis (commensal strain). After 24-h incubation, S. aureus predominated in the binary community, but later it was replaced by S. epidermis. Norepinephrine at higher concentrations accelerated this process without principally changing it. The model can be used to develop more effective complex antimicrobial drugs.

摘要

提出了一种简单高效的方法，可在模拟鼻腔天然微环境的海藻酸钠凝胶基质中获得单种和双种金黄色葡萄球菌和表皮葡萄球菌培养物。使用这些培养物来研究去甲肾上腺素对两种细菌（金黄色葡萄球菌（侵袭株）和表皮葡萄球菌（共生株）单种和双种群落的影响。孵育 24 小时后，双种群落中金黄色葡萄球菌占优势，但后来被表皮葡萄球菌取代。较高浓度的去甲肾上腺素加速了这一过程，但并没有从根本上改变这一过程。该模型可用于开发更有效的复杂抗菌药物。

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本文引用的文献

1
Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models.三维器官型皮肤模型中的宿主-微生物相互作用和针对微生物组的治疗方法的新方法。
Microbiome. 2023 Oct 17;11(1):227. doi: 10.1186/s40168-023-01668-x.
2
and : Two Major Sentinels of Skin Microbiota and the Influence of Cosmetics.以及：皮肤微生物群的两大主要哨兵及化妆品的影响
Microorganisms. 2020 Nov 7;8(11):1752. doi: 10.3390/microorganisms8111752.
3
Efficacy of antiseptics in a novel 3-dimensional human plasma biofilm model (hpBIOM).
新型三维人血浆生物膜模型（hpBIOM）中防腐剂的功效。
Sci Rep. 2020 Mar 16;10(1):4792. doi: 10.1038/s41598-020-61728-2.
4
An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics.一种体外胶原灌注伤口生物膜模型；可应用于抗菌研究和微生物代谢组学。
BMC Microbiol. 2019 Dec 30;19(1):310. doi: 10.1186/s12866-019-1682-5.
5
3D bioprinting of mature bacterial biofilms for antimicrobial resistance drug testing.三维生物打印成熟细菌生物膜用于抗菌药物耐药性检测。
Biofabrication. 2019 Sep 13;11(4):045018. doi: 10.1088/1758-5090/ab37a0.
6
QuPath: Open source software for digital pathology image analysis.QuPath：用于数字病理学图像分析的开源软件。
Sci Rep. 2017 Dec 4;7(1):16878. doi: 10.1038/s41598-017-17204-5.
7
Colonization, pathogenicity, host susceptibility, and therapeutics for Staphylococcus aureus: what is the clinical relevance?金黄色葡萄球菌的定植、致病性、宿主易感性和治疗：有何临床相关性？
Semin Immunopathol. 2012 Mar;34(2):185-200. doi: 10.1007/s00281-011-0300-x. Epub 2011 Dec 11.
8
Hair follicles as a niche of Staphylococcus aureus in the nose; is a more effective decolonisation strategy needed?毛囊作为鼻腔内金黄色葡萄球菌的生态位：是否需要更有效的去定植策略？
J Hosp Infect. 2010 Nov;76(3):211-4. doi: 10.1016/j.jhin.2010.07.011.
9
An in vitro model of bacterial infections in wounds and other soft tissues.一种在体外模拟伤口和其他软组织细菌感染的模型。
APMIS. 2010 Feb;118(2):156-64. doi: 10.1111/j.1600-0463.2009.02580.x.
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Catecholamine inotropes as growth factors for Staphylococcus epidermidis and other coagulase-negative staphylococci.儿茶酚胺类强心药作为表皮葡萄球菌和其他凝固酶阴性葡萄球菌的生长因子。
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