拉伸蛋白质以了解生物学：最新进展。
Understanding biology by stretching proteins: recent progress.
机构信息
Instituto Cajal, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
出版信息
Curr Opin Struct Biol. 2010 Feb;20(1):63-9. doi: 10.1016/j.sbi.2010.01.003. Epub 2010 Feb 6.
Abstract
Single molecule manipulation techniques combined with molecular dynamics simulations and protein engineering have enabled, during the last decade, the mechanical properties of proteins to be studied directly, thereby giving birth to the field of protein nanomechanics. Recent data obtained from such techniques have helped gain insight into the structural bases of protein resistance against forced unfolding, as well as revealing structural motifs involved in mechanical stability. Also, important technical developments have provided new perspectives into protein folding. Eventually, new and exciting data have shown that mechanical properties are key factors in cell signaling and pathologies, and have been used to rationally tune these properties in a variety of proteins.
摘要
在过去的十年中，单分子操纵技术结合分子动力学模拟和蛋白质工程，使人们能够直接研究蛋白质的机械性能，从而诞生了蛋白质纳力学领域。这些技术获得的最新数据有助于深入了解蛋白质抵抗强制展开的结构基础，并揭示了参与机械稳定性的结构基序。此外，重要的技术发展为蛋白质折叠提供了新的视角。最终，令人兴奋的新数据表明，机械性能是细胞信号转导和病理学的关键因素，并已被用于合理调节各种蛋白质的这些特性。
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本文引用的文献
1
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2
Mechanical unfolding of an ankyrin repeat protein.肌动蛋白重复蛋白的机械展开。
Biophys J. 2010 Apr 7;98(7):1294-301. doi: 10.1016/j.bpj.2009.12.4287.
3
Non-native interactions are critical for mechanical strength in PKD domains.非天然相互作用对 PKD 结构域的机械强度至关重要。
Structure. 2009 Dec 9;17(12):1582-1590. doi: 10.1016/j.str.2009.09.013.
4
Constructing the equilibrium ensemble of folding pathways from short off-equilibrium simulations.从短时间的偏离平衡模拟中构建折叠途径的平衡系综。
Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19011-6. doi: 10.1073/pnas.0905466106. Epub 2009 Nov 3.
5
Mechanical strength of 17,134 model proteins and cysteine slipknots.17134 种模型蛋白质和半胱氨酸滑结的机械强度。
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6
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7
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8
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