无标记生物标志物在悬浮微通道谐振器中的未稀释血清中的感应。
Label-free biomarker sensing in undiluted serum with suspended microchannel resonators.
机构信息
Department of Biological Engineering and Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
Anal Chem. 2010 Mar 1;82(5):1905-10. doi: 10.1021/ac9027356.
Abstract
Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded microchannels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO(2) surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL.
摘要
需要改进常规、廉价的生物标志物监测方法，以便更早地发现和描述癌症。悬浮微通道谐振器（SMR）是高度灵敏、批量制造的微悬臂梁，具有嵌入式微通道，可以通过谐振频率的变化直接定量吸附的质量。与其他无标记检测方法一样，由于血清等复杂介质中存在非特异性结合的高背景信号，生物分子测量具有挑战性。在本报告中，我们证明了为直接吸附到 SMR SiO(2)表面而开发的羧基甜菜碱衍生聚合物可作为超低污染和功能化的表面涂层。与参考微悬臂梁结合，这种方法能够在未稀释的血清中检测到激活的白细胞细胞黏附分子（ALCAM），这是一种模型癌症生物标志物，检测限为 10 ng/mL。
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