Kohwang Medical Research Institute, School of Medicine, Kyung Hee University, Seoul 130-701, Korea.
Korean J Physiol Pharmacol. 2008 Jun;12(3):125-9. doi: 10.4196/kjpp.2008.12.3.125. Epub 2008 Jun 30.
The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of matrix metallopeptidase 2 (MMP2) are associated with obesity. MMP2 is an enzyme with proteolytic activity against matrix and nonmatrix proteins, particularly basement membrane constituents. To identify the relationship between polymorphisms of MMP2 and overweight/obese, we genotyped 5 SNPs (rs17242319, rs1053605, rs243849, rs2287074, and rs10775332) of the coding region of MMP2 using the Golden Gate assay on an Illumina BeadStation 500 GX. One hundred and forty two overweight/obese (BMI >/=23) and 145 normal (BMI 18 to <23) subjects were analyzed. SNPStats, Haploview, HapAnalyzer, SNPAnalyzer, and Helixtree programs were used for the analysis of genetic data. A linkage disequilibrium (LD) block was discovered among the 5 SNPs selected, including rs17242319, rs1053605, rs243849, and rs2287074. Of the 5 polymorphisms, 2 synonymous SNPs [rs17242319 (Gly226Gly) and rs10775332 (Phe602Phe)] were found significant associations with overweight/obese. Recently, rs1132896 replaced rs17242319 as a new number (SNP database, BUILD 129). In haplotype analysis using Haploview, a haplotype (haplotype: CCCA) containing a meaningful polymorphism (rs17242319) was found to be significantly different. The results suggest that MMP2 may be associated with overweight/obese in Korean population.
本研究旨在确定基质金属蛋白酶 2（MMP2）的单核苷酸多态性（SNP）是否与肥胖有关。MMP2 是一种具有蛋白水解活性的酶，可针对基质和非基质蛋白，特别是基底膜成分。为了确定 MMP2 多态性与超重/肥胖的关系，我们使用 Golden Gate assay 技术在 Illumina BeadStation 500 GX 上对 MMP2 编码区的 5 个 SNP（rs17242319、rs1053605、rs243849、rs2287074 和 rs10775332）进行了基因分型。分析了 142 名超重/肥胖（BMI≥23）和 145 名正常体重（BMI 18 至<23）受试者。使用 SNPStats、Haploview、HapAnalyzer、SNPAnalyzer 和 Helixtree 程序对遗传数据进行分析。在所选的 5 个 SNP 中发现了一个连锁不平衡（LD）块，包括 rs17242319、rs1053605、rs243849 和 rs2287074。在这 5 种多态性中，发现 2 个同义 SNP [rs17242319（Gly226Gly）和 rs10775332（Phe602Phe）]与超重/肥胖显著相关。最近，rs1132896 取代 rs17242319 成为新的编号（SNP 数据库，BUILD 129）。在使用 Haploview 的单体型分析中，发现一个含有有意义多态性（rs17242319）的单体型（单体型：CCCA）存在显著差异。结果表明，MMP2 可能与韩国人群中的超重/肥胖有关。
