College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Poult Sci. 2010 Mar;89(3):464-9. doi: 10.3382/ps.2009-00461.
Infectious bronchitis virus (IBV), the causative agent of infectious bronchitis, results in respiratory disease, nephritis, and poor egg production and quality in chicken. Antimicrobial peptides possess potent antimicrobial activities and are regarded as promising therapeutic alternatives in the fight against microorganisms. To assess the in vitro antiviral activity of swine intestine antimicrobial peptides (SIAMP) against IBV, 45 chick embryos were randomly assigned into 3 groups, 15 for each group. Embryos in group 1 were inoculated with IBV. Group 2 was inoculated with SIAMP-IBV intermixture. Group 3 was used as a control and inoculated with sterile PBS. Allantoic fluid was collected for hemagglutination titer assay. In addition, weight gain, mortality, and pathological changes for each group were recorded. The results showed that no distinct pathological changes were found in chick embryos after they were inoculated with SIAMP-IBV intermixture and the mortality was reduced remarkably compared with the IBV-infected group. Weight gain of embryos in the SIAMP-IBV intermixture group was significantly higher than the IBV-infected embryos (P < 0.01), which was also higher than the control group (P < 0.05). Furthermore, the hemagglutination titer in the SIAMP-IBV group was significantly lower than that in the IBV-infected group (P < 0.01). These results indicated that SIAMP can inhibit virus replication and reduce tissue injury caused by IBV.
传染性支气管炎病毒（IBV）是传染性支气管炎的病原体，可导致鸡呼吸道疾病、肾炎以及产蛋量和蛋品质下降。抗菌肽具有强大的抗菌活性，被认为是对抗微生物的有前途的治疗替代品。为了评估猪肠抗菌肽（SIAMP）对 IBV 的体外抗病毒活性，将 45 枚鸡胚随机分为 3 组，每组 15 枚。第 1 组鸡胚接种 IBV，第 2 组接种 SIAMP-IBV 混合物，第 3 组作为对照接种无菌 PBS。收集所有囊液用于血凝滴度测定。此外，记录每组的增重、死亡率和病理变化。结果显示，接种 SIAMP-IBV 混合物的鸡胚无明显病理变化，死亡率明显低于 IBV 感染组。SIAMP-IBV 混合物组的胚胎增重明显高于 IBV 感染组（P < 0.01），也高于对照组（P < 0.05）。此外，SIAMP-IBV 组的血凝滴度明显低于 IBV 感染组（P < 0.01）。这些结果表明，SIAMP 可以抑制病毒复制并减轻 IBV 引起的组织损伤。
