Youssef Diaa T A, Ibrahim Amany K, Khalifa Sherief I, Mesbah Mostafa K, Mayer Alejandro M S, van Soest Rob W M
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Nat Prod Commun. 2010 Jan;5(1):27-31.
Bioassay-guided fractionation of the anti-inflammation fractions of the Red Sea sponges Scalarispongia aqabaensis and Callyspongia siphonella yielded two new sterols from chloroform fractions of methanol extracts, namely scalaristerol (5alpha,8alpha-dihydroxycholest-6-en-3beta-ol) (1) from Scalarispongia aqabaensis, and callysterol (ergosta-5,11-dien-3beta-ol) (2) from Callyspongia siphonella. Structure determination was based on extensive NMR studies and mass spectrometry. The antiinflammatory activity of compounds 1 and 2 was assessed using the rat-hind paw edema method and by study of their effect on the release of O2(-) and TXB2 from LPS-activated rat neonatal microglia.
对红海海绵阿喀巴标枪海绵（Scalarispongia aqabaensis）和虹吸管钙质海绵（Callyspongia siphonella）的抗炎组分进行生物测定导向的分级分离，从甲醇提取物的氯仿级分中得到了两种新的甾醇，即从阿喀巴标枪海绵中得到的标枪甾醇（5α,8α - 二羟基胆甾 - 6 - 烯 - 3β - 醇）（1），以及从虹吸管钙质海绵中得到的钙质甾醇（麦角甾 - 5,11 - 二烯 - 3β - 醇）（2）。结构测定基于广泛的核磁共振研究和质谱分析。使用大鼠后爪水肿法并通过研究化合物1和2对脂多糖激活的大鼠新生小胶质细胞释放O2(-)和TXB2的影响，评估了它们的抗炎活性。
