Freeman R, Gould F K, Wilkinson R, Ward A C, Lightfoot N F, Sisson P R
Department of Microbiology, University of Newcastle upon Tyne, UK.
Epidemiol Infect. 1991 Apr;106(2):239-46. doi: 10.1017/s095026880004838x.
Pyrolysis mass spectrometry (PyMS) was used as a method of rapid inter-strain comparison of 19 isolates of coagulase-negative staphylococci from episodes of CAPD peritonitis. Thirteen isolates were from multiple, but distinct, episodes of peritonitis in 6 patients and the remaining 6 isolates were from 6 patients with single episodes. The results, expressed in terms of identity/non-identity of strains, were compared with those obtained using an established typing system comprising an extended antibiogram, determination of biotype and plasmid profile analysis. The PyMS results for inter-strain comparison were in agreement with the reference typing scheme results. PyMS can be used in this setting to rapidly obtain evidence that persistent infection is/is not likely to be due to the same organism, although it cannot be used for formal typing. The results by both methods showed that serial, apparently distinct, episodes of peritonitis over periods as long as 120 days may be due to the same strain of coagulase-negative staphylococcus. Clinically based distinctions between recurrence of infection (same strain) and re-infection (different strains) may not always be supported by the microbiological evidence.
热解质谱法（PyMS）被用作一种对19株来自持续性非卧床腹膜透析（CAPD）腹膜炎发作的凝固酶阴性葡萄球菌进行菌株间快速比较的方法。13株菌株来自6例患者的多次但不同的腹膜炎发作，其余6株菌株来自6例单次发作的患者。以菌株的相同/不同表示的结果，与使用包括扩展抗菌谱、生物型测定和质粒图谱分析的既定分型系统所获得的结果进行了比较。菌株间比较的PyMS结果与参考分型方案结果一致。在这种情况下，PyMS可用于快速获得证据，证明持续性感染是否可能由同一生物体引起，尽管它不能用于正式分型。两种方法的结果均表明，长达120天的一系列明显不同的腹膜炎发作可能是由同一株凝固酶阴性葡萄球菌引起的。基于临床对感染复发（同一菌株）和再感染（不同菌株）的区分可能并不总是得到微生物学证据的支持。
