International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong 515031, PR China.
Virology. 2010 May 25;401(1):1-5. doi: 10.1016/j.virol.2010.02.024. Epub 2010 Mar 19.
A lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus.
流感病毒 PB2 蛋白第 627 位的赖氨酸（627K）已被认为是宿主适应性和一些流感病毒毒力的决定因素。虽然季节性流感病毒仅含有 627K，但大流行性（H1N1）2009 病毒具有谷氨酸（627E），即使在人类中传播了 6 个多月。为了探讨 PB2 中 E627K 取代在大流行性（H1N1）2009 病毒中的潜在作用，我们比较了含有 627K PB2 基因的重组病毒与含有 627E 的亲本 A/California/4/2009 株之间的致病性和生长特性。我们的结果表明，PB2 基因中 627K 的取代并没有赋予大流行性（H1N1）2009 病毒在小鼠和细胞培养中更高的毒力和生长速度，这表明 627K 不是大流行性（H1N1）2009 病毒适应人类的必需条件。
